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| Costa,Bruna Zucoloto da; Rodrigues,Viviane Drumond; Oliveira,Valéria Maia de; Ottoboni,Laura Maria Mariscal; Marsaioli,Anita Jocelyne. |
| Abstract Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine, Canaã dos Carajás, Brazil). Hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. Bacterial strains with good oxidative performance were also evaluated for biotransformation of organic sulfides. Fourteen strains with good enzymatic activity were identified by 16S rRNA gene sequencing, revealing the presence of three genera: Bacillus, Pseudomonas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Copper mine drainage; High-throughput screening; Hydrolases; Monooxygenases. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400846 |
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| Stoppe,Nancy de Castro; Silva,Juliana Saragiotto; Torres,Tatiana Teixeira; Carlos,Camila; Hachich,Elayse Maria; Sato,Maria Inês Zanoli; Saraiva,Antonio Mauro; Ottoboni,Laura Maria Mariscal. |
| Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B2(3) in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: E. coli; Phylogenetic groups; Pollution sources; Interaction networks; Social network analysis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500012 |
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