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Gene Expression Analysis: A Way to Study Tolerance to Abiotic Stresses in Crops Species Chilean J. Agric. Res.
Pérez-Torres,Eduardo; Paredes C,Mario; Polanco,Víctor; Becerra B,Viviana.
Regions traditionally destined to agriculture report an ever increasing exposure to cold and drought conditions. This is especially important in countries like Chile where crop management options are limited. The development of new cultivars with better yields under adverse conditions is fundamental if the ever increasing demand for food is to be matched; however, improving tolerance to abiotic stresses has proved to be a complex task. In this regard, development in plant physiology and genomics in the last 20 years has led to a deeper understanding of how plants respond to stress and mechanisms responsible for different ranges of tolerance observed in nature. This review discusses the techniques currently most in use in gene expression analysis, together...
Tipo: Journal article Palavras-chave: Cold; Drought; Crops species; Gene expression.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200016
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Effect of a commercial pectinmethylesterase on tomato paste consistency Electron. J. Biotechnol.
Zúñiga-Hansen,María Elvira; Pérez-Torres,Eduardo; Caballero-Valdés,Eduardo; Fernández-Parodi,José.
Background: Consistency is one of the main traits that define commercial quality and price of tomato paste. Pectins are partially responsible for consistency in tomato paste, therefore enzymatic pectin modification could be used to increase paste consistency. Results: This work reports the effects of a commercial enzymatic preparation of pectin-methyl-esterase (PME) (NovoShape™) on tomato paste consistency taking into account variables as enzyme/substrate ratio (0,1% w/w - 1% w/w), reaction time (0 hr - 3 hrs) and reaction temperature (40ºC-60ºC). The results indicate that NovoShape™ increased consistency when reaction temperature ranged from 40 to 50ºC with an enzyme/substrate ratio of 0.5 to 1 (l PME solution/g tomato paste on dry base). On the other...
Tipo: Journal article Palavras-chave: Pectin methyl esterase; Tomato paste; Viscosity.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300009
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