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MOLECULAR EXCLUSION CHROMATOGRAPHY OF CRUDE VENOM AS AN AUXILIARY TOOL TO IDENTIFY HYBRID HONEYBEE POPULATIONS J. Venom. Anim. Toxins
PALMA,M.S.; BROCHETTO-BRAGA,M.R.; CHAUD-NETTO,J.; MALASPINA,O.; OLIVEIRA,M.R..
A comparison among the profiles of molecular exclusion chromatography in Sephadex G 100 column of venoms from Apis mellifera adansonii and Africanized honeybees revealed unique peaks which might be used to identify these populations. The venoms from hybrid populations resulting from the reciprocal mating of Apis mellifera adansonii and Africanized honeybees presented unique peaks, probably resulting from a synergistic effect between the parental genomes. The occurrence of characteristic peaks in venoms of hybrid populations might be used to identify these populations as well as to distinguish them from their parents.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Venom; Hybrid populations; Molecular exclusion chromatography; Honeybees.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000100004
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ALARM PHEROMONES AND THE INFLUENCE OF PUPAL ODOR ON THE AGGRESSIVENESS OF Polybia paulista (IHERING) (HYMENOPTERA: VESPIDAE) J. Venom. Anim. Toxins
MANZOLI-PALMA,M.F.; GOBBI,N.; PALMA,M.S..
Field bioassays were used to demonstrate that aggressive behavior of Polybia paulista (Ihering) workers is elicited by alarm pheromones present in the venom reservoirs of nest defenders and that the brood care pheromone (pupal odor) produced by the young inside the nest also plays an important defensive role. Pupal odor was extracted from the surface of pupa bodies with methanol. When bioassayed alone, the pupal odor elicited only attractiveness of workers towards the odor source, but no stinging attacks were observed. However, in the presence of alarm pheromones, the brood care pheromone potentiated the effect caused by the pupal odors, increasing the number of stinging attacks during an action of colony defense. Thus, the presence of pupae within the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Wasps; Brood; Defense behavior; Venom reservoirs.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100006
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THE EFFECT OF A POLYDNAVIRUS OF APANTELES GALLERIAE ON THE HEMOLYMPH PROTEINS OF HOST GALLERIA MELLONELA J. Venom. Anim. Toxins
BROCHETTO-BRAGA,M.R.; PALMA,M.S.; RIBEIRO,J.C.; GOBBI,N..
The presence of a polydnavirus in the ovary of the microhymenoptera Apanteles galleriae was detected. This wasp parasites the larvae of the wax moth Galleria mellonella. To study the possible viral effects on the total protein of G.mellonella, hemolymph extracts of the ovary calyxes of parasitized A. galleriae were injected into 6th and 7th instar cells of G.mellonella larvae. Protein profiles on polyacrilamide gel electrophoresis containing sodium dodecyl sulfate (SDS-PAGE) of host cell extracts showed a suppression of several protein bands when infection occurred in the 6th instar. However, in the 7th instar, parasitism caused an amplification in the synthesis of most proteins with some changes in the electrophoretic profile.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microhymenoptera; Wax moth; Parasitism; Polydnavirus; SDS-PAGE; Apanteles galleriae; Galleria mellonela.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000200005
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BIOINSECTICIDE ACTION OF THE VENOM OF AFRICANIZED BEES Apis mellifera LINNAEUS 1758 (HYMENOPTERA: APIDAE): I -THE MOST SUSCEPTIBLE AGE OF Diatraea saccharalis FABRICIUS 1794 (LEPELETIER: PYRALIDAE) EGGS TO THE VENOM ACTION J. Venom. Anim. Toxins
BONFANTI-ALMEIDA,J.C.; GOBBI,N.; PALMA,M.S..
The present paper aimed at testing the action of non-lyophilized venom of Africanized bees Apis mellifera through topical applications on Diatraea saccharalis egg masses. The CL50, DL50 and the most susceptible age of eggs to the venom topic application were also determined. Three-day-old eggs were the most susceptible to the venom action with CL50 equal to 8.6 mg/ml and DL50 equal to 0.173 mg/mass. The venom loses its action after being stored for 15 days.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Venom; Africanized bee; Apis mellifera; Eggs; Sugar-cane borer; Diatraea saccharalis.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100006
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CHEMICAL COMMUNICATION IN Apis mellifera: TEMPORAL MODULATION OF ALARM BEHAVIORS J. Venom. Anim. Toxins
COSTA,H.; TALORA,D. C.; PALMA,M.S.; CHAUD-NETTO,J..
The effect of pheromones and their chemical analogues in honeybee alarm behaviors was studied in observation boxes. Defensive behaviors, as follows: a) attraction to scent source, b) elevation of wings in "V", c) abdomen elevation, d) abdomen elevation and pumping and e) first leg pair elevation had been temporarily registered when the following compounds were presented: isoamyl alcohol, octyl alcohol, benzyl alcohol, n-butyl acetate, n-octyl acetate, isopentyl acetate, benzyl acetate and 2-heptanone. The results were as follows: 1. the bees elicited some characteristic behaviors when chemical alarm messages were presented, 2. agression (stinging) was not completed with any compound tested, probably because there was not a target (visual stimulus), 3. in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Defensive behavior; Alarm behaviors; Pheromones; Hybrid honey bees; Chemical communication.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100005
Registros recuperados: 5
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