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Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent Biol. Res.
GARCÍA,CARLOS; PEREIRA,PAULO; VALLE,LUIS; LAGOS,NÉSTOR.
Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disease caused by lipid soluble polyether toxins produced by dinoflagellates and accumulated in shellfish. Diarrhetic shellfish poisoning is a worldwide threat to public health and the shellfish industry. To date, only four lipid soluble polyethers have been known as diarrhetic shellfish toxins. Among them, Okadaic acid (OA), Dinophysistoxin 1 (DTX-1, 35-methyl OA), Dinophysistoxin 2 (DTX-2, OA isomers) and Dinophysistoxin 3 (DTX-3, 7-O-acyl-35-methyl OA), all of which have free carboxilic groups. To perform quantitative analysis of DSP toxins in shellfish samples is a requirement, because DSP toxins are endemic in the Chilean mollusks of the southern regions, and although human symptoms of DSP...
Tipo: Journal article Palavras-chave: DSP toxins; Okadaic acid; Dinophysistoxin-1 (DTX-1); Pyrenyldiazomethane (PDAM); Patagonian fjords; Chile.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200007
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Toxic cyanobacteria strains isolated from blooms in the Guadiana River (southwestern Spain) Biol. Res.
MORENO,ISABEL M; PEREIRA,PAULO; FRANCA,SUSANA; CAMEÁN,ANA.
This paper describes the occurrence of toxic cyanobacteria along the Guadiana River over its course between Mérida and Badajoz (Extremadura, Spain). Water sampling for phytoplankton quantification and toxin analysis was carried out regularly between 1999 and 2001 in six different locations, including two shallow, slow-flowing river sites, two streamed river sites and two drinking water reservoirs. The cyanobacterial community differed significantly between these locations, especially during the summer. The predominant genera were Microcystis, Oscillatoria, Aphanizomenon and Anabaena. Using an ELISA assay the total microcystin contents of natural water samples from the most eutrophic locations ranged from 0.10 _ 21.86 mg mcyst-LR equivalent·L-1 in...
Tipo: Journal article Palavras-chave: Cyanobacteria; Microcystins; Guadiana; Spain.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300006
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