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	Provedor de dados BJMBR (2) Autor Paçó-Larson,M.L. (2) Monesi,N. (1) Pereira,G.B. (1) Sousa,J.F. (1) Valente,V. (1) Vianna,M.C.B. (1) de Queiroz,M.S. (1) Mais... Palavra-chave Aberrant splicing (1) DNA puff (1) Development (1) DmLpin (1) Ecdysone (1) Gene expression (1) Insertional mutagenesis (1) Kermit/dGIPC (1) Transgenic Drosophila (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster BJMBR Pereira,G.B.; Valente,V.; de Queiroz,M.S.; Vianna,M.C.B.; Paçó-Larson,M.L.. Insertional mutagenesis is an important tool for functional genomics in Drosophila melanogaster. The insertion site in the KG00562 mutant fly line has been mapped to the CG8709 (herein named DmLpin) locus and to the 3’ of kermit (also called dGIPC). This mutant line presents a high lethality rate resulting from a gain of function. To obtain some insight into the biological role of the mutated locus, we have characterized the mutation and its relation to the high mortality of the KG00562 fly line. In this mutant, we did not detect one of the DmLpin transcripts, namely DmLpinK, but we did detect an unusual 2.3-kb mRNA (LpinK-w). Further investigation revealed that the LpinK-w transcript results from an aberrant splicing between the untranslated first exon of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insertional mutagenesis; Aberrant splicing; DmLpin; Kermit/dGIPC. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400003 The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila BJMBR Monesi,N.; Sousa,J.F.; Paçó-Larson,M.L.. We extended the characterization of the DNA puff BhB10-1 gene of Bradysia hygida by showing that, although its mRNA is detected only at the end of the fourth larval instar, BhB10-1 expression is not restricted to the salivary gland, the tissue in which this gene is amplified. Different amounts of BhB10-1 mRNA were detected in other larval tissues such as gut, Malpighian tubules, fat body, brain and cuticle, suggesting that this gene is expressed differentially in the various tissues analyzed. Analysis of transgenic Drosophila carrying the BhB10-1 transcription unit and flanking sequences revealed that the tested fragment promotes transcription in a constitutive manner. We suggest that either cis-regulatory elements are missing in the transgene or factors... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA puff; Gene expression; Development; Ecdysone; Transgenic Drosophila. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700003 Registros recuperados: 2 Primeira ... 1 ... Última

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