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| Lerin,Lindomar; Toniazzo,Geciane; Oliveira,Débora de; Rottava,Leda; Dariva,Cláudio; Cansian,Rogério Luis; Treichel,Helen; Padilha,Francine; Antunes,Octávio Augusto Ceva. |
| Limonene is a monoterpene obtained in large amounts from essential oils and is used as a raw material for the synthesis of flavors and fine chemicals. Several pathways or routes for the microbial degradation of limonene making use of the cytochrome P450-dependent monooxygenases have been described. In this study, we present a fermentative screening of microorganisms in order to verify their ability to perform the desirable conversion. In parallel, the PCR technique was used to select the microorganisms that contain the limC gene, which is responsible for the conversion of carveol to carvone. The microorganisms selected by PCR were not able to bioconvert limonene. From this result, we can suppose that these strains do not have the gene that codifies the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDH (carveol dependent dehydrogenase); Carveol; Carvone; PCR; Fermentative screening. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200017 |
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