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Endophytic Actinomycetes as Potential Producers of Hemicellulases and Related Enzymes for Plant Biomass Degradation BABT
Robl,Diogo; Mergel,Carla Montanari; Costa,Patricia dos Santos; Pradella,José Geraldo da Cruz; Padilla,Gabriel.
Abstract Tailor made enzymatic preparation must be design to hydrolyze efficiently plant biomass, once that each plant biomass possesses a distinct cell wall composition. Most of actinomycetes used for plant cell wall degradation are focused on the cellulases and xylanases production. However, a wide range of enzymes must be produced for an efficient degradation of lignocellulose materials. During the last decade several unusual environments were studied to obtain strains that produce glycohydrolases with innovator characteristics. In this context, the present work concerned the selection of endophytic actinomycetes as producers of hemicellulases and related enzymes with different enzymatic profiles, for use in the deconstruction of lignocellulosic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic actinomycetes; Hemicellulases; Accessory enzymes.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100207
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New thermal inducible Streptomyces phages isolated from tropical soils Braz. J. Genet.
Balan,Andrea; Padilla,Gabriel.
Two new Streptomyces phages, øBP1 and øBP2, were isolated from tropical soil samples. These phages presented a large host range and developed both lytic and lysogenic responses in different Streptomyces species tested. Variations in the incubation temperature showed to be important in the development of the replication cycle. Increasing incubation temperature from 30oC to 42oC induced the lytic response of øBP2 and lysogenic of øBP1 in the host strain Streptomyces sp. WL6. øBP1 and øBP2 have icosahedral heads with long tails and were characterized in relation to morphology, G + C content, genome size and adsorption curve
Tipo: Info:eu-repo/semantics/article
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400002
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The function of lexical motifs in the organization of the Actinomycetes 5S rRNAs BJM
Rodrigues-Subacius,Sandra M; Padilla,Gabriel.
This work shows results obtained by employing the linguistic method to identify biologically meaningful sites in Actinomycetes 5S rRNAs. The approach adopted identifies triplet-words, along the base sequence of 5S rRNA, located mainly at the alpha and beta domains of the 5S secondary structure. There are triplet-words representing universal protein binding sites that include important prokaryote signatures, and sites strategically located in critical regions related to the formation of the 5S ribonucleoproteins (RNP) complex. In those sites, where the GC pressure promoted substitutions, the analysis demonstrates that alterations did not affect their biological significance. Sites formed by GGY (or more rarely GGR), continued to play an important role as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycetes; 5S rRNA; GC pressure; Linguistic analysis; Lexical motifs.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300036
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Carbon catabolite repression of retamycin production by Streptomyces olindensis ICB20 BJM
Inoue,Olavo Ossamu; Schmidell Netto,Willibaldo; Padilla,Gabriel; Facciotti,Maria Cândida Reginato.
Retamycin is an anthracyclinic antitumoral complex produced by Streptomyces olindensis ICB20. In this work the influence of different glucose concentrations in the feed medium on the production of retamycin was studied. Chemostat cultures employing glucose concentration varying between 10 g/L and 25 g/L showed that use of high glucose concentration resulted in catabolite repression of the biosynthesis of the antitumoral. The highest specific retamycin production rate, qRTM = 7.8 mg/g.h, was obtained when glucose concentration was 10 g/L. The lowest value of qRTM, 2.5 mg/g.h, was observed when glucose concentration was 20 and 25 g/L. The residual glucose concentration varied from 0 to 13 g/L, as the glucose concentration in the feed was increased from 10 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Anthracyclines; Carbon catabolite repression.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100012
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