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Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers Genet. Mol. Biol.
Paduan,Karina dos Santos; Araújo-Júnior,João P.; Ribolla,Paulo E.M..
The tropical mosquito, Aedes aegypti is the most important domestic vector of urban yellow fever and dengue. Genetic population studies on this vector are important because they may lead to new tools for surveillance. An analysis of genetic structure was conducted among populations of A. aegypti from 11 localities in four demographic regions within six Brazilian federal states. Markers included 21 random amplified polymorphic DNA (RAPD) loci. RAPD markers were detected among populations and cluster analysis revealed two main groups. We found high genetic polymorphism (H S = 0.224) and high levels of genetic differentiation between populations from different states (G ST = 0.430), as well as in populations from cities in the same state (G ST = 0.410). These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Population genetics; Random amplified polymorphic DNA; Genetic variability.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200030
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Human respiratory syncytial virus detection in children admitted at a community hospital in Botucatu, SP, Brazil BJM
Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais.
Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015
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