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	Provedor de dados J. Venom. Anim. Toxins incl. Trop. Dis. (2) Autor Paiva-Cavalcanti,M (2) Brandão-Filho,SP (1) Brito,MEFC (1) Gomes,YM (1) Gonçalves,SC (1) Regis-da-Silva,CG (1) Régis-da-Silva,CG (1) Mais... Palavra-chave Diagnosis (2) Glyceraldehyde-3-phosphate dehydrogenase (1) Leishmaniasis (1) Molecular diagnosis (1) Quality control (1) Visceral leishmaniasis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis J. Venom. Anim. Toxins incl. Trop. Dis. Gonçalves,SC; Régis-da-Silva,CG; Brito,MEFC; Brandão-Filho,SP; Paiva-Cavalcanti,M. Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (G3PD) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. The systems created for G3PD recognition were combined with detection systems for L. infantum and L.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Diagnosis; Glyceraldehyde-3-phosphate dehydrogenase; Quality control. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200009 Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review J. Venom. Anim. Toxins incl. Trop. Dis. Paiva-Cavalcanti,M; Regis-da-Silva,CG; Gomes,YM. In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Molecular diagnosis; Visceral leishmaniasis. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400004 Registros recuperados: 2 Primeira ... 1 ... Última

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