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	Provedor de dados BABT (1) Autor Banerjee,Rintu (1) Palit,Soumen (1) Palavra-chave Alpha- amylase (1) Bacillus circulans (1) Extraction (1) Solid state fermentation (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2001 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313 BABT Palit,Soumen; Banerjee,Rintu. The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus circulans; Solid state fermentation; Alpha- amylase; Extraction. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015 Registros recuperados: 1 Primeira ... 1 ... Última

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