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	Provedor de dados BJMBR (2) Autor Panek,A.D. (2) Paschoalin,V.M.F. (2) Silva,J.T. (2) De Mesquita,J.F. (1) Rolim,M.F. (1) Souza,A.C. (1) de Araujo,P.S. (1) Mais... Palavra-chave Trehalase (2) CAMP-dependent protein kinase (1) CaM kinase II (1) Calmodulin (1) Candida utilis (1) Saccharomyces cerevisiae (1) Sugar transporter (1) Trehalose (1) YLR270W (1) Yeast (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evidence for a modulation of neutral trehalase activity by Ca2+ and cAMP signaling pathways in Saccharomyces cerevisiae BJMBR Souza,A.C.; De Mesquita,J.F.; Panek,A.D.; Silva,J.T.; Paschoalin,V.M.F.. Saccharomyces cerevisiae neutral trehalase (encoded by NTH1) is regulated by cAMP-dependent protein kinase (PKA) and by an endogenous modulator protein. A yeast strain with knockouts of CMK1 and CMK2 genes (cmk1cmk2) and its isogenic control (CMK1CMK2) were used to investigate the role of CaM kinase II in the in vitro activation of neutral trehalase during growth on glucose. In the exponential growth phase, cmk1cmk2 cells exhibited basal trehalase activity and an activation ratio by PKA very similar to that found in CMK1CMK2 cells. At diauxie, even though both cells presented comparable basal trehalase activities, cmk1cmk2 cells showed reduced activation by PKA and lower total trehalase activity when compared to CMK1CMK2 cells. To determine if CaM kinase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Trehalase; Calmodulin; CaM kinase II; CAMP-dependent protein kinase; YLR270W. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100002 Shared control of maltose and trehalose utilization in Candida utilis BJMBR Rolim,M.F.; de Araujo,P.S.; Panek,A.D.; Paschoalin,V.M.F.; Silva,J.T.. Trehalose biosynthesis and its hydrolysis have been extensively studied in yeast, but few reports have addressed the catabolism of exogenously supplied trehalose. Here we report the catabolism of exogenous trehalose by Candida utilis. In contrast to the biphasic growth in glucose, the growth of C. utilis in a mineral medium with trehalose as the sole carbon and energy source is aerobic and exhibits the Kluyver effect. Trehalose is transported into the cell by an inducible trehalose transporter (K M of 8 mM and V MAX of 1.8 µmol trehalose min-1 mg cell (dry weight)-1. The activity of the trehalose transporter is high in cells growing in media containing trehalose or maltose and very low or absent during the growth in glucose or glycerol. Similarly, total... Tipo: Info:eu-repo/semantics/article Palavras-chave: Trehalose; Trehalase; Sugar transporter; Candida utilis; Yeast. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700002 Registros recuperados: 2 Primeira ... 1 ... Última

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