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Loreto,Elgion L.S.; Deprá,Maríndia; Diesel,José F.; Panzera,Yanina; Valente-Gaiesky,Vera Lucia S.. |
Abstract Hypermutable strains of Drosophila simulans have been studied for 20 years. Several mutants were isolated and characterized, some of which had phenotypes associated with alteration in development; for example, showing ectopic legs with eyes being expressed in place of antennae. The causal agent of this hypermutability is a non-autonomous hobo-related sequence (hoboVA). Around 100 mobilizable copies of this element are present in the D. simulans genome, and these are likely mobilized by the autonomous and canonical hobo element. We have shown that hoboVA has transcription factor binding sites for the developmental genes, hunchback and even-skipped, and that this transposon is expressed in embryos, following the patterns of these genes. We suggest... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hobo; Transposable elements; Cis-regulatory sequences; Drosophila. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200198 |
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Degrandi,Tiago Marafiga; Pita,Sebastian; Panzera,Yanina; Oliveira,Edivaldo Herculano C. de; Marques,José Ribamar Felipe; Figueiró,Marivaldo Rodrigues; Marques,Larissa Coêlho; Vinadé,Lucia; Gunski,Ricardo José; Garnero,Analía Del Valle. |
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 18S rDNA; Bubalus bubalis; FISH. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300009 |
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