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Genetic similarity among male bees of Euglossa truncata Rebêlo & Moure (Hymenoptera: Apidae) revealed by RAPD markers Neotropical Entomology
Suzuki,Karen M.; Almeida,Fernanda S.; Sodré,Leda M.K.; Pascual,Amália N.T.; Sofia,Silvia H..
The genetic RAPD (random amplified polymorphic DNA) markers have been used successfully in taxonomical studies of several groups of organisms. In the present study these molecular markers were used to analyze the genetic similarity among eighteen males of Euglossa truncata Rebêlo & Moure exhibiting variations in two morphological characters (colour of the antennal scape and metaepisternal hairs) which are frequently used to identify this species of euglossine bee. The twelve primers used in the RAPD analysis amplified 127 loci, of which 40 (31.5%) were polymorphic, showing some variation among the individuals. The coefficients of genetic similarity among the individuals ranged from 0.79 to 0.95, indicating a rather high genetic similarity among the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoidea; Euglossini; Orchid bee; Euglossine bee; Molecular marker.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000400008
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Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina) Neotropical Entomology
Pascual,Amália N.T.; Suzuki,Karen M.; Almeida,Fernanda S.; Sodré,Leda M.K.; Sofia,Silvia H..
In the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P < 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoidea; Euglossini; Orchid bee; Euglossine bee; Molecular marker.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014
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