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Hepatitis E virus seroprevalence among schistosomiasis patients in Northeastern Brazil BJID
Passos-Castilho,Ana Maria; de Sena,Anne; Domingues,Ana Lucia Coutinho; Lopes-Neto,Edmundo Pessoa; Medeiros,Tibério Batista; Granato,Celso Francisco Hernandez; Ferraz,Maria Lúcia.
Abstract Background Hepatitis E virus (HEV) can cause chronic infection with rapid progression to liver cirrhosis in immunocompromised patients. HEV seroprevalence in patients with Schistosoma mansoni in Brazil is unknown. We evaluated the prevalence of past or present HEV infection in schistosomiasis patients in Recife, Pernambuco, Brazil. A total of 80 patients with Schistosoma mansoni were consecutively enrolled in a cross-sectional study. Serum samples were tested for the presence of anti-HEV IgG antibodies by enzyme immunoassay (Wantai anti-HEV IgG, Beijing, China) and for the presence of HEV RNA using real time reverse transcriptase-polymerase chain reaction with primers targeting the HEV ORF2 and ORF3. Clinical and laboratory tests as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Epidemiology; Hepatitis E virus; Schistosomiasis; Seroprevalence.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300007
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High prevalence of hepatitis E virus antibodies in Sao Paulo, Southeastern Brazil: analysis of a group of blood donors representative of the general population BJID
Passos-Castilho,Ana Maria; Reinaldo,Mônica Renata; Sena,Anne de; Granato,Celso F.H..
Abstract Brazil is a non-endemic country for hepatitis E virus (HEV) infection with seroprevalence from 1% to 4% in blood donors and the general population. However, data on seroprevalence of HEV in the country are still limited. This study evaluated the prevalence of past or present HEV infection in a group of blood donors representative of the general population of the city of Sao Paulo, Southeastern Brazil. Serum samples from 500 blood donors were tested from July to September 2014 by serological and molecular methods. Anti-HEV IgG antibodies were detected in 49 (9.8%) subjects and categorized age groups revealed an age-dependent increase of HEV seroprevalence. Among the anti-HEV IgG positive subjects, only 1 had anti-HEV IgM while none tested positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis E virus; Blood donors; Seroprevalence; Brazil.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500535
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High frequency of hepatitis E virus infection in swine from South Brazil and close similarity to human HEV isolates BJM
Passos-Castilho,Ana Maria; Granato,Celso Francisco Hernandes.
Abstract Hepatitis E virus is responsible for acute and chronic liver infections worldwide. Swine hepatitis E virus has been isolated in Brazil, and a probable zoonotic transmission has been described, although data are still scarce. The aim of this study was to investigate the frequency of hepatitis E virus infection in pigs from a small-scale farm in the rural area of Paraná State, South Brazil. Fecal samples were collected from 170 pigs and screened for hepatitis E virus RNA using a duplex real-time RT-PCR targeting a highly conserved 70 nt long sequence within overlapping parts of ORF2 and ORF3 as well as a 113 nt sequence of ORF2. Positive samples with high viral loads were subjected to direct sequencing and phylogenetic analysis. hepatitis E virus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Genotype 3; Hepatitis E virus; Pigs; Zoonosis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200373
Registros recuperados: 3
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