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| Bhushan,Bharat; Patra,Biswa Nath; Das,Pranab Jyoti; Dutt,Triveni; Kumar,Pushpendra; Sharma,Arjava; Umang,; Dandapat,Satyabarta; Ahlawat,Shiv Pal Singh. |
| The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: MHC; BoLa-A polymorphism; Cattle. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400009 |
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