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| Rungsa,Prapenpuksiri; Incamnoi,Paroonkorn; Sukprasert,Sophida; Uawonggul,Nunthawun; Klaynongsruang,Sompong; Daduang,Jureerut; Patramanon,Rina; Roytrakul,Sittiruk; Daduang,Sakda. |
| Abstract Background: Wasp venom is a complex mixture containing proteins, enzymes and small molecules, including some of the most dangerous allergens. The greater banded wasp (Vespa tropica) is well-known for its lethal venom, whose one of the major components is a hyaluronidase (HAase). It is believed that the high protein proportion and activity of this enzyme is responsible for the venom potency. Methods: In the present study, cDNA cloning, sequencing and 3D-structure of Vespa tropica venom HAase were described. Anti-native HAase antibody was used for neutralization assay. Results: Two isoforms, VesT2a and VesT2b, were classified as members of the glycosidase hydrolase 56 family with high similarity (42–97 %) to the allergen venom HAase. VesT2a gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wasp venom; Vespa tropica; Hyaluronidase (HAase). |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100321 |
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| Rungsa,Prapenpuksiri; Janpan,Piyapon; Saengkun,Yutthakan; Jangpromma,Nisachon; Klaynongsruang,Sompong; Patramanon,Rina; Uawonggul,Nunthawun; Daduang,Jureerut; Daduang,Sakda. |
| Abstract Background: Crude venom of the banded tiger waspVespa affinis contains a variety of enzymes including hyaluronidases, commonly known as spreading factors. Methods: The cDNA cloning, sequence analysis and structural modelling of V. affinis venom hyaluronidase (VesA2) were herein described. Moreover, heterologous expression and mutagenesis of rVesA2 were performed. Results: V. affinis venom hyaluronidase full sequence is composed of 331 amino acids, with four predicted N-glycosylation sites. It was classified into the glycoside hydrolase family 56. The homology modelling exhibited a central core (α/β)7 composed of Asp107 and Glu109, acting as the catalytic residues. The recombinant protein was successfully expressed in E. coli with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vespa affinis Hyaluronidase Wasp; Venom Structure analysis Modelling Cloning Protein expression. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100321 |
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