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| Husted, Søren; Persson, D.P.; Laursen, K.H.; Hansen, T.H.; Pedas, P.; Schiller, M.; Hegelund, J.N.; Schjørring, J.K.. |
| Inductively coupled plasma-mass spectrometry (ICP-MS) is the state-of-the-art technique for multi-elemental analysis of plant tissue. It provides a powerful tool in functional genomics, linking altered elemental profiles of mutants with gene expression and function. In addition, with its unmatched sensitivity, ICP-MS enables characterization of the substrate specificity and regulation of membrane transport proteins. Digestion of plant tissue has traditionally represented a bottleneck due to the low capacity of commercially available equipment. However, recent developments in micro-scaled digestion, combined with semi-quantitative analysis and chemometrics, have enabled high-throughput multi-elemental profiling and multivariate classification of large... |
| Tipo: Journal paper |
Palavras-chave: Food systems; Knowledge management. |
| Ano: 2011 |
URL: http://orgprints.org/19089/1/Review.pdf |
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| Hansen, T.H.; de Bang, T.C.; Laursen, K.H.; Pedas, P.; Husted , S.; Schjoerring , J.K.. |
| Plant tissue analysis is a valuable tool for evaluating the nutritional status and quality of crops and is widely used for scienti fi c and commercial purposes. The majority of plant analyzes are now performed by techniques based on ICP spectrometry such as inductively coupled plasma—optical emission spectroscopy (ICP-OES) or ICP—mass spectrometry (ICP-MS). These techniques enable fast and accurate measurements of multielement pro fi les when combined with appropriate methods for sample preparation and digestion. This chapter presents state-of-the-art methods for digestion of plant tissues and subsequent analysis of their multielement composition by ICP spectrometry. Details on upcoming techniques, expected to gain importance within the fi eld of... |
| Tipo: Book chapter |
Palavras-chave: Technology transfer Education; Extension and communication. |
| Ano: 2013 |
URL: http://orgprints.org/22588/1/Chapter%208.pdf |
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