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Freitas,Mariana Ramos; Freire,Naiana Braga; Peixoto,Luciana Jatobá e Silva; Oliveira,Samira Teixeira Leal de; Souza,Renilde Cordeiro de; Gouveia,João José de Simoni; Costa,Mateus Matiuzzi da; Gouveia,Gisele Veneroni. |
ABSTRACT: Aeromonas hydrophila is a common fish pathogen that causes extensive damage to aquaculture. To develop and implement a more adequate strategy to farm fish, it is crucial to understand the bacterial-resistance levels and their transference dynamics. The objective of this study was to analyze the resistance profile of isolated Aeromonas hydrophila to antimicrobial agents and heavy metals and draw a correlation of the observed profiles with the presence of plasmids. Resistance of the isolated bacteria to antimicrobial agents (oxacilin, gentamicin, tetracycline, and nalidixic acid) and heavy metals (cadmium, lead, copper, and manganese) was verified using the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquiculture; Multiresistance; Megaplasmids; Cromossomic DNA; Integrons. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900451 |
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Peixoto,Rodolfo de Moraes; Araújo,Renata de Moraes Peixoto; Peixoto,Luciana Jatobá e Silva; Reges,Ariel Marques; Alves,Ana Paula Pereira; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido; Azevedo,Sérgio Santos; Costa,Mateus Matiuzzi da. |
ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial culture; Intramammary infection; Small ruminant; Somatic cell count. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217 |
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