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| Freitas,Vicente J.F.; Serova,Irina A.; Andreeva,Lyudmila E.; Dvoryanchikov,Guennadi A.; Lopes-Jr.,Edilson S.; Teixeira,Dárcio I.A.; Dias,Luciene P.B.; Avelar,Suely R.G.; Moura,Raylene R.; Melo,Luciana M.; Pereira,Alexsandra F.; Cajazeiras,João B.; Andrade,Maria L.L.; Almeida,Karlliely C.; Sousa,Francisco C.; Carvalho,Antonio C.C.; Serov,Oleg L.. |
| In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 adult undefined breed goats were used as donors and recipients, respectively. Donors were estrus-synchronized with vaginal sponges and superovulated by a treatment with 200 mg FSH given twice daily in decreasing doses over 3 days starting 48 h before sponge removal. Ovulation was induced by injecting 100µg GnRH 36 h after sponge removal. The recipients also received an estrus synchronization treatment. Donors were mated with fertile Saanen bucks and, approximately 72 h after sponge removal, zygotes were recovered surgically by flushing oviducts. The recovered zygotes were briefly centrifuged to a reliable visualization of the pronuclei. The DNA construct containing hG-CSF... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenesis; Goat; HG-CSF; DNA microinjection; Embryo. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400003 |
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