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| Costa,E.P.; Lopes,F.G.; Pereira,E.C.M.; Queiroz,V.L.D.; Macedo,G.G.; Almeida Neto,J.R.M.; Costa,A.H.A.. |
| A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of counting. The process was done after embryo fixation in a sodium citrate solution, and adherence in glass slide. Following, the coloration was done using a fast panoptic coloration kit. As a result, it was possible to identify the blastomeres and count them in each blastocyst. This method provided a fast and effective analysis of the total cell number when compared with other techniques. Moreover, this... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Contagem de células; Embrião; Reprodução. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600032 |
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| Grázia,J.G.V.; Silveira,R.O.; Pereira,E.C.M.; Santos,G.M.. |
| RESUMO O Brasil atualmente é detentor do terceiro maior rebanho de vacas leiteiras do mundo, composto, em sua maioria, de animais mestiços F1 (Gir X Holandês), os quais são bem adaptados às áreas tropicais. O objetivo do presente estudo foi investigar a eficiência da ovum pick-up (OPU) e da produção in vitro de embriões oriundos de doadoras Girolando com sêmen sexado de touros provenientes de duas raças, Gir e Holandesa. Foram utilizados dados referentes a 232 animais de diversos rebanhos, totalizando 4334 oócitos recuperados. Os oócitos foram classificados, e as estruturas viáveis (GI, GII e GIII) foram utilizadas para produção in vitro de embriões (PIVE). Foi observada uma média de oócitos recuperados para as doadoras F1 de 18,14±1,33. Além disso,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Girolando; OPU; PIVE. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300605 |
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| Sollecito,N.V.; Pereira,E.C.M.; Grázia,J.G.V.; Neves,B.P.; Couto,B.V.R.; Andrade,V.B.; Miranda,M.S.; Silva,J.K.R.; Borges,A.M.. |
| ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Apoptosis; Lippia origanoides; In vitro embryo production. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723 |
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