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| Betancur,Gabriel J. Vargas; Pereira Jr,Nei. |
| Sugar cane bagasse is produced in Brazil as waste of the sugar and ethanol industries. This lignocellulosic material is a potential source for second-generation ethanol production; however a pretreatment stage is essential, which aims at removing the hemicellulose component by disorganizing the lignocellulosic complex. In this work sugar cane bagasse was pretreated by diluted acid hydrolysis resulting in xylose-rich hydrolysates, which could be fermented to ethanol by a strain of the yeast Pichia stipitis. Statistical approach was used to investigate the effects of factors associated with the diluted acid hydrolysis process (acid concentration, solid:liquid ratio and time of exposure) on the fermentability of different hydrolysates. The statistical... |
| Tipo: Journal article |
Palavras-chave: Bioethanol; Pichia stipitis; Xylose fermentation. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500014 |
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| Vargas Betancur,Gabriel J; Pereira Jr,Nei. |
| Tons of sugar cane bagasse are produced in Brazil as waste of the sugar and ethanol industries. This lignocellulosic material is a potential source for second-generation ethanol production. Diluted acid hydrolysis is one of the most efficient pretreatments for hemicellulosic solubilization. The hydrolysate obtained is rich in xylose, which can be converted to ethanol by Pichia stipitis. This work used a statistical approach and the severity factor to investigate the effects of factors associated with the diluted acid hydrolysis process (acid concentration, solid:liquid ratio and time of exposure) on various response variables (xylose concentration, hydrolysis yield, inhibitor concentration and hydrolysate fermentability). The severity factor had a strong... |
| Tipo: Journal article |
Palavras-chave: Hemicellulose hydrolysis; Severity factor; Xylose. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300010 |
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| Ferreira,Verônica; Faber,Mariana de Oliveira; Mesquita,Sabrina da Silva; Pereira Jr,Nei. |
| In Brazil, the production of ethanol from sugarcane produces large amounts of lignocellulosic residues (bagasse and straw), which have been driving research and development for the production of second generation ethanol. In the present work, a recombinant Saccharomyces cerevisiae strain expressing the β-glucosidase gene from Humicola grisea was used for ethanol production from three different cellulosic sources by simultaneous saccharification and fermentation. Initially, a enzymatic pre-hydrolysis step was done with a solid:liquid ratio of 1:4, and an enzymatic load of 25 filter paper activity (FPU).g-1 of cellulosic substrate. Using sugarcane bagasse pretreated cellulignin, crystalline cellulose and carboxymethyl cellulose, 51.7 g L-1, 41.7 g... |
| Tipo: Journal article |
Palavras-chave: Bioethanol; Lignocellulosic biomass; Sugarcane bagasse derived cellulignin. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200005 |
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| Damaso,Mônica Caramez Triches; Andrade,Carolina M.M. Carvalho; Pereira Jr,Nei. |
| In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylanases; Thermomyces lanuginosus; Corncob; Submerged fermentation; Medium optimization. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
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