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| Humily, Florian; Farrant, Gregory K.; Marie, Dominique; Partensky, Frederic; Mazard, Sophie; Perennou, Morgan; Labadie, Karine; Aury, Jean-marc; Wincker, Patrick; Segui, Audrey Nicolas; Scanlan, David J.; Garczarek, Laurence. |
| Synechococcus, one of the most abundant cyanobacteria in marine ecosystems, displays a broad pigment diversity. However, the in situ distribution of pigment types remains largely unknown. In this study, we combined flow cytometry cell sorting, whole-genome amplification, and fosmid library construction to target a genomic region involved in light-harvesting complex (phycobilisome) biosynthesis and regulation. Synechococcus community composition and relative contamination by heterotrophic bacteria were assessed at each step of the pipeline using terminal restriction fragment length polymorphism targeting the petB and 16S rRNA genes, respectively. This approach allowed us to control biases inherent to each method and select reliable WGA products to construct... |
| Tipo: Text |
Palavras-chave: Marine cyanobacteria; Phycobilisomes; Whole-genome amplification; Flow cytometry cell sorting; Fosmid library. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00374/48495/48843.pdf |
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| Jouault, Albane; Gobet, Angelique; Simon, Marjolaine; Portier, Emilie; Perennou, Morgan; Corre, Erwan; Gaillard, Fanny; Vallenet, David; Michel, Gurvan; Fleury, Yannick; Bazire, Alexis; Dufour, Alain. |
| The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue... |
| Tipo: Text |
Palavras-chave: Antibiofilm protein; Pseudoalteromonas; Pseudomonas aeruginosa; Biofilm. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75517/76405.pdf |
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| Lemonnier, Clarisse; Perennou, Morgan; Eveillard, Damien; Fernandez-guerra, Antonio; Leynaert, Aude; Marié, Louis; Morrison, Hilary G.; Memery, Laurent; Paillard, Christine; Maignien, Lois. |
| Ocean frontal systems are widespread hydrological features defining the transition zone between distinct water masses. They are generally of high biological importance as they are often associated with locally enhanced primary production by phytoplankton. However, the composition of bacterial communities in the frontal zone remains poorly understood. In this study, we investigate how a coastal tidal front in Brittany (France) structures the free-living bacterioplankton communities in a spatio-temporal survey across four cruises, five stations and three depths. We used 16S rRNA gene surveys to compare bacterial community structures across 134 seawater samples and defined groups of co-varying taxa (modules) exhibiting coherent ecological patterns across... |
| Tipo: Text |
Palavras-chave: Marine front; Bacterial communities; Dynamic; Network; Ecological strategies. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00634/74657/74557.pdf |
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| Gobet, Angelique; Mest, Laetitia; Perennou, Morgan; Dittami, Simon M.; Caralp, Claire; Coulombet, Celine; Huchette, Sylvain; Roussel, Sabine; Michel, Gurvan; Leblanc, Catherine. |
| Background: Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host. In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized. Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass. Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet. We investigated the effect of four... |
| Tipo: Text |
Palavras-chave: Microbe-host interactions; Digestive microbiota; Abalone; Macroalgae; Holobiont. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00489/60071/63368.pdf |
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