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	Provedor de dados BJID (6) BJM (2) Autor Perez,Leandro Reus Rodrigues (8) Barth,Afonso Luís (3) d'Azevedo,Pedro Alves (3) Antunes,Ana Lúcia Souza (2) Caierão,Juliana (1) Cantarelli,Vlademir Vicente (1) Costi,Ricardo (1) Dias,Cícero Armídio Gomes (1) Dias,Cícero Gomes (1) Freitas,Ana Lúcia Peixoto de (1) Limberger,Mariana Fagundes (1) Rodrigues,Diógenes (1) Santos,Juliana Conrad dos (1) Secchi,Carina (1) Weber,Liliana Portal (1) Mais... Palavra-chave Pseudomonas aeruginosa (3) Biofilm (2) Cefoxitin (2) Cystic fibrosis (2) Oxacillin (2) Antimicrobial resistance (1) Auxotrophy (1) Carbapenems (1) Clindamycin (1) CoNS (1) Coagulase-negative Staphylococcus (1) D test (1) Enterobacteriaceae (1) Flow cytometry (1) KPC (1) MecA gene (1) Metallo-β-lactamase (1) Phenotypic tests (1) Resistance (1) Surveillance (1) Mais... Tipo do documento Info:eu-repo/semantics/article (7) Info:eu-repo/semantics/other (1) Ano 2015 (1) 2014 (1) 2011 (2) 2008 (2) 2007 (2) País Brazil (8) Idioma Inglês (8)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 8 Primeira ... 1 ... Última Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units BJID Perez,Leandro Reus Rodrigues; Rodrigues,Diógenes; Dias,Cícero Gomes. In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight selective enrichment broth containing 10 µg ertapenem disk followed by culture on MacConkey agar. A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. Carbapenem-resistant Enterobacteriaceae was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed... Tipo: Info:eu-repo/semantics/other Palavras-chave: Carbapenems; Enterobacteriaceae; KPC; Surveillance. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400436 Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci BJID Secchi,Carina; Antunes,Ana Lúcia Souza; Perez,Leandro Reus Rodrigues; Cantarelli,Vlademir Vicente; d'Azevedo,Pedro Alves. The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coagulase-negative Staphylococcus; Cefoxitin; Oxacillin; MecA gene; Susceptibility diagnostic. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012 Use of the D test method to detect inducible clindamycin resistance in coagulase negative staphylococci (CoNS) BJID Perez,Leandro Reus Rodrigues; Caierão,Juliana; Antunes,Ana Lúcia Souza; d'Azevedo,Pedro Alves. According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method to evaluate the inducible clindamycin resistance in accordance with an approach of the disks of erythromycin and clindamycin - the D test - has been reported. We analyzed the performance of this method in 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures of hospitalized patients at a general hospital in Southern Brazil. Twenty-seven clinical isolates with suitable profile (erythromycin-resistant and clindamycin-susceptible) were evaluated for the D test realization. Thus, only 5 CoNS were D test positive. The D test method showed to be simple and an important technique in the detection of inducible clindamycin resistance. Tipo: Info:eu-repo/semantics/article Palavras-chave: Clindamycin; Resistance; D test. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200002 Evaluation of urinalysis parameters to predict urinary-tract infection BJID Santos,Juliana Conrad dos; Weber,Liliana Portal; Perez,Leandro Reus Rodrigues. We evaluated the performance of automated-flow cytometry, urinalysis dipsticks and microscopic urine sediment analysis as predictors of urinary tract infection. Urine cultures were used as a reference method for comparison. Six-hundred-seventy-five urine samples from hospitalized and not hospitalized patients attended at Hospital Mãe de Deus, Porto Alegre, in south Brazil, were included in the study. Among the individual measures analyzed, intense bacteriuria in the microscopic analysis of urinary sediment gave an accuracy of 92.9%. A combination between intense bacteriuria (microscopic analysis) and >20 leukocytes per µL of urine (flow cytometry) gave a higher accuracy (97.3%). We conclude that though it is laborious, microscopic urinalysis is a good... Tipo: Info:eu-repo/semantics/article Palavras-chave: Urinary tract infection; Urinalysis; Flow cytometry. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500008 Biofilm production using distinct media and antimicrobial susceptibility profile of Pseudomonas aeruginosa BJID Perez,Leandro Reus Rodrigues; Barth,Afonso Luís. Biofilm production is an important mechanism for bacterial survival and its occurrence together with antimicrobial resistance represents a challenge for clinical management. Here, we evaluated the ability for biofilm production among P. aeruginosa isolates from patients with or without cystic fibrosis (CF) using two distinct media, besides determining the antimicrobial susceptibility profile of these isolates for eight antimicrobial agents. The ability for biofilm production when TSB medium was used was higher than when used CF sputum media (p = 0.0198). However, P. aeruginosa isolates from CF have demonstrated similar performance for biofilm production, independently of the medium used. Besides, among the biofilm-producing isolates, those recovered from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Pseudomonas aeruginosa; Cystic fibrosis; Antimicrobial resistance. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400001 Evaluation of the accuracy of various phenotypic tests to detect oxacillin resistance in coagulase-negative staphylococci BJID Perez,Leandro Reus Rodrigues; d'Azevedo,Pedro Alves. In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30µg-cefoxitin and 1µg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. Of these, 138 (78.4%) harbored the mecA gene. Using 30µg-cefoxitin and 1µg-oxacillin disks we obtained 100 and 98.3% accuracy, respectively. In addition, when cefoxitin was used as marker in an agar dilution method, the higher accuracy (99.4%) was established with 8mg cefoxitin per mL breakpoint. Thus, despite of the agar dilution method using cefoxitin as a marker not being... Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxacillin; Cefoxitin; CoNS. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300009 Nutritional requirement among Pseudomonas aeruginosa isolates recovered from respiratory clinical specimens at a tertiary hospital from South of Brazil BJM Perez,Leandro Reus Rodrigues; Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luís. We screened 349 isolates of P. aeruginosa from cystic fibrosis (CF+) and non-cystic fibrosis (CF-) patients for the auxotrophy. Fourteen (4.0%) were auxotrophic and among them only one was recovered from CF-patient showing that this characteristic is strongly associated with cystic fibrosis. In total, a requirement for 5 different compounds (or combination) was verified and, of these, methionine was the most common single amino acid required. Only one auxotrophic isolate was no able to produce biofilm in vitro. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Auxotrophy; Cystic fibrosis; Biofilm. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300026 Evaluation of tests to predict metallo-β-lactamase in cystic fibrosis (CF) and non-(CF) Pseudomonas BJM Perez,Leandro Reus Rodrigues; Limberger,Mariana Fagundes; Costi,Ricardo; Dias,Cícero Armídio Gomes; Barth,Afonso Luís. Double disks synergy test (DDST) and combined disks test (CD) were evaluated to predict the presence of metallo-β-lactamase in 70 Pseudomonas aeruginosa isolates recovered from cystic fibrosis and non-cystic fibrosis patients. DDST CAZ-EDTA 1 cm and CD IMP-EDTA tests showed the best accuracy (94.3%). Furthermore, for other combinations, accuracy unsatisfactory was obtained. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Metallo-β-lactamase; Phenotypic tests. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300011 Registros recuperados: 8 Primeira ... 1 ... Última

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