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Sorgeloost, P.; Baeza-mesa, M.; Benijts, F.; Bossuyt, E.; Bruggeman, E.; Claus, C.; Persoone, G.; Van De Putte, G.; Versichele, D.. |
In view of the need'for research on the practical use of the brine shrimp Artemia salina in aquaaulture, our research team at the State University of Ghent (Belgium) is studying the following aspects : 1- The automatized mass-culturing of larvae. 2- The controlled production of cysts. 3- The comparative study of geographical strains. 4- The study of the metabolism of the cysts. |
Tipo: Text |
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Ano: 1977 |
URL: http://archimer.ifremer.fr/doc/00305/41626/40835.pdf |
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Vanhaecke, P.; Persoone, G.; Claus, C.; Sorgeloos, P.. |
Although standardization of toxicity tests on aquatic organisms is an urgent necessity, very little has yet been achieved for the marine environment. As a first step in this direction, a simple, inexpensive and reliable short-term routine test with <i>Artemia</i> larvae is proposed. This test is the result of an extensive study in our laboratory, taking into account the advantages and disadvantages of <i>Artemia</i> bioassays published by various authors. The major advantage of the brine shrimp as a test species is its continuous availability under the form of dry cysts which can be hatched very easily; this eliminates all biological, technological, and financial problems of stock recruitment and/or culturing. The acute test... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nauplii; Toxicity tests; Artemia. |
Ano: 1981 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3711 |
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Persoone, G.. |
The displacement technique for measuring the volume of plankton catches is well established, and a number of papers have been published describing suitable apparatus for this purpose including those by Frolander (1957), and Yentsch & Hebard (1957). Gnanamuthu (1952) reviews several papers dealing with equipment for measuring the volume of small aquatic animals and discusses his own volumeter. The apparatus here described employs a water column and is, therefore, more convenient to handle than previous designs which used a mercury column. It is inexpensive and simple to construct, and will measure to 0.01 or even 0.005 ccm. We have found it most useful in quickly measuring the volume of polychaetes or oligochaetes, small bivalves, small crustaceans, etc. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Instruments; Marine invertebrates. |
Ano: 1971 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3430 |
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Sorgeloos, P.; Persoone, G.. |
The 3 culturing devices described are based on the principle of the classic air-water lift, which facilitates continuous recirculation and aeration of the cultivation medium. The first device is a "circulation cylinder", convenient for hatching eggs of the brine shrimp <i>Artemia salina</i> and for culturing algae and protozoans. In the second device, an internal basket (i.e., a glass cylinder with plankton-gauze bottom) prevents the animals being carried along in the air-water lift. The third device, analogous to the second, is provided with a lateral waste-drain and a water collector with siphon, which empties regularly into the main culture vessel. The second and third devices proved to be excellent for the cultivation of a variety of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Fish larvae; Instruments; Marine invertebrates. |
Ano: 1972 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3461 |
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Persoone, G.. |
Predictive hazard assessment of chemicals, pure, in mixtures, or as wastes, is based on the comparison of exposure to effect concentrations. Experimental determination of no-effect levels can be carried out at various levels of biological organisation: within the organism (suborganismal), on individual species (organismal), or with biological communities (supraorganismal). The difficulty of predictive effect analysis consists of relating endpoints determined at various levels of biological organisation to the impact on structure and/or function impairment of natural ecosystems. Examples are given of effect-extrapolations which have been validated at different levels, and which carry promising predictive values. |
Tipo: Info:eu-repo/semantics/article |
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Ano: 1988 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3154 |
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Vanhaecke, P.; Persoone, G.; Claus, C.; Sorgeloos, P.. |
Standardization of toxicity tests on aquatic organisms to detect the impact of chemicals on freshwater or marine ecosystems is an urgent necessity. From the variety of methods, criteria, and test species proposed, simple standardized tests for algae, crustaceans, and fish are now close to being adopted at the international level for the freshwater environment. Despite the same urgency, very little has been achieved for the marine environment. <i>Artemia</i> is an extremely suitable test-species since, contrary to all other organisms, it does not require continuous maintenance of stock cultures. Unfortunately none of the <i>Artemia</i> toxicity tests that have been worked out so far can be adopted as such as a representative standard... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nauplii; Toxicity tests; Artemia. |
Ano: 1980 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3698 |
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Persoone, G.; Morales, J.; Verlet, H.; De Pauw, N.. |
Since turbulence is an important factor for the growth of microscopic algae, the effect of mixing intensity and mixing time, has been assessed by means of air-lift pumps as an alternative technology for the agitation of algal cultures. Batch culture experiments in enriched sea water were carried out indoors with <i>Chlorella</i> in 100 liter tanks and outdoors with natural phytoplankton populations in 70 m³ tanks. The respective culturing depths in these tanks were 25 and 180 cm. The role of the mixing was also evaluated against that of the pH regulation of the cultures. Mixing with air-lift pumps increased the algal growth rate significantly in comparison to non-agitated cultures. The yields were also much higher (up to 30%) in mixed than in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algae culture. |
Ano: 1980 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3692 |
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Vanhaecke, P.; Persoone, G.. |
Considering the necessity for standardization of toxicity tests and the need for simple and reliable routine tests, the <i>Artemia</i> Reference Center at the State University of Ghent in Belgium has developed a short term standard toxicity test with <i>Artemia</i> nauplii. This simple and inexpensive bioassay which can be used as a screening test to determine the toxicity of chemicals to aquatic organisms, has been discussed in detail during a special workshop devoted to toxicity tests with brine shrimp, at the occasion of the "International Symposium on the brine shrimp <i><i>Artemia</i> salina</i>"(Corpus Christi, Texas, USA; August 1979). Besides a few minor modifications, the test methodology was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intercalibration; Nauplii; Toxicity tests; Artemia. |
Ano: 1981 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3377 |
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Persoone, G.; Sorgeloos, P.. |
Starting with the major issues of the recent World Conference on Aquaculture, organized in 1981 jointly by the European Mariculture Society and the World Mariculture Society, the paper indicates a number of new and appealing maricultural technologies in shellfish and fish farming. It is postulated that several of the techniques described will have a far-reaching impact on maricultural production in the next decade. Special attention is given to the potential of the mass production of brine shrimp <i>Artemia</i> in extensive and intensive operations, and in particular to its key role in vertically integrated aquaculture. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture techniques; Literature reviews. |
Ano: 1982 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3385 |
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Persoone, G.. |
The oyster culture, flourishing along the Belgian coast at the beginning of the century, perished entirely in the course of two World Wars. In 1956, it was taken on again intensively in the Scour Basin of Ostend. If we consider the obtained results with the affination of Flat and Portuguese oyster, this 86 ha closed basin, is of an exceptional productivity. Essays on the understanding of the origin of calcified oysters, done from 1960 until 1966, turned out to be very promising and an integral oyster culture from the larvae up to the adult oyster could be observed. In any case the last few years, eventhough compared to before a normal emission of larvae of <i>Ostrea edulis</i>, there has not been a fixation of the origin. Regular samples of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brackishwater pollution Harbours Oyster culture Reproduction Ostrea edulis Linnaeus; 1758 ANE; Belgium; Oostende Harbour; Sluice Dock. |
Ano: 1971 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3467 |
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Persoone, G.; Deplaecie, M.. |
During ecological investigations on the optimum culturing conditions of ciliates, we studied the influence of a number of abiotic factors on the reproductive potential of the hypotrichous ciliate <i>Euplotes vannus</i> Muller, such as the nature and the quantity of food, the temperature and the salinity. The experiments were run in petri dishes of 5 cm diameter containing 5 ml of food suspension, and were always started with conjugants. The microphagous <i>Euplotes vannus</i> thrives on a variety of food media. Excellent results were observed on homogenized bakers yeast, i.e. 10.000-20.000 animals in 5 ml of food suspension. In good culturing conditions the stationary phase was reached in 7 days, which implicates a mean rate of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reproduction Euplotes vannus ANE; Belgium; Oostende Harbour. |
Ano: 1972 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3468 |
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Sorgeloos, P.; Baeza-Mesa, M.; Benijts, F.; Persoone, G.. |
In the framework of research on mariculture at the "Institute for Marine Scientific Research" (IZWO), the "Laboratory for Biological Research in Environmental Pollution" at the State University of Ghent, is focusing on culturing problems of the brine shrimp <i>Artemia salina</i>. The following research topics are discussed: 1. The mechanisms leading to the hatching of <i>Artemia</i> cysts are reviewed and complemented with new data on the sensitivity to light and temperature, and on the reversibility of the development within the cyst. The possible influences of the collecting, sorting, and packaging processes on the quality of the cysts are discussed. 2. Since in the literature much confusion exists concerning the mechanisms... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 1976 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3533 |
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Sorgeloos, P.; Remiche-Van der Wielen, C.; Persoone, G.. |
Because of their easy hatching from dry cysts and their year-round availability, nauplii of the brine shrimp <i><i>Artemia</i> salina</i> are most convenient test organisms for toxicity studies. The bioassays mentioned in literature have, however, mostly been carried out with larvae of which neither the exact instar stage nor the geographical origin is known. The incubation of the cysts should always be carried out under strictly controlled temperature conditions since both the onset of hatching and the length of the hatching period are temperature-dependent. Moreover, the experiments should only be carried out with populations of nauplii of the same stage of development since it is demonstrated that the second and third instars are... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nauplii; Toxicity tests; Artemia. |
Ano: 1978 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3588 |
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Sorgeloos, P.; Persoone, G.. |
Although extensive fundamental research has been performed on the brine shrimp, the yields from hatching and culturing this organism which is widely used in mariculture, can be greatly improved. By exposing the immersed cysts to light and by using a new type of hatching cylinder and separator box, greater hatching and separation efficiencies can be obtained. Controlled mass culture of brine shrimp larvae to the adult stage has been extremely difficult until now; the literature on the subject is reviewed and a new technique of high density culturing at the pilot-stage is described. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artemia salina. |
Ano: 1975 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3511 |
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Persoone, G.; Sorgeloos, P.. |
1. A simple cylindrical hatching and separator box is described for <i>Artemia salina</i>nauplii (and other phototactic invertebrates), in which the "corner seeking effect" responsible for poor separation is avoided, and from which nauplii can be recovered very conveniently. 2. Separation is achieved by partly unscrewing an internal and closed cylinder from its fixed counter-part glued to the bottom of the box, till two slits are freed through which the nauplii migrate from the darkened internal hatching cylinder into the illuminated exterior compartment. Rescrewing the former cylinder separates the larvae from debris and unhatched eggs. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hatching; Instruments; Nauplii; Phototaxis; Artemia. |
Ano: 1972 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3460 |
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Registros recuperados: 50 | |
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