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	Provedor de dados BJM (2) Autor Farías,Jorge G. (2) Figueroa,Carolina A. (2) Pessoa,Adalberto (2) Zepeda,Andrea B. (2) Rabert,Claudia (1) Weinacker,Daniel (1) Mais... Palavra-chave Yeast (2) Heterologous expression system (1) Lipid peroxidation (1) Production (1) Pyruvate pathway (1) Recombinant protein (1) Scale up (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2013 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris BJM Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G.. ABSTRACT The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate; and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris. We evaluated cell viability using propidium iodide by flow cytometry and thiobarbituric acid reactive substances to measure cell membrane damage. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipid peroxidation; Pyruvate pathway; Yeast. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400856 Applications of recombinant Pichia pastoris in the healthcare industry BJM Weinacker,Daniel; Rabert,Claudia; Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G.. Since the 1970s, the establishment and development of the biotech industry has improved exponentially, allowing the commercial production of biopharmaceutical proteins. Nowadays, new recombinant protein production is considered a multibillion-dollar market, in which about 25% of commercial pharmaceuticals are biopharmaceuticals. But to achieve a competitive production process is not an easy task. Any production process has to be highly productive, efficient and economic. Despite that the perfect host is still not discovered, several research groups have chosen Pichia pastoris as expression system for the production of their protein because of its many features. The attempt of this review is to embrace several research lines that have adopted Pichia... Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Heterologous expression system; Recombinant protein; Production; Scale up. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400004 Registros recuperados: 2 Primeira ... 1 ... Última

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