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Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels BJM
Phutela,Urmila; Dhuna,Vikram; Sandhu,Shobhna; Chadha,B.S..
A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50ºC. Highest enzyme activities of 1116 Ug-1 for pectinase and 1270 Ug-1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermophilic fungi; Pectinase; Polygalacturonase.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100013
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