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| Pichard,Gastón. |
| There is little information known about the response of cup-plant (Silphiumperfoliatum L.), a perennial forage crop introduced to Chile, to differing cultivation practices. A series of studies was conducted to evaluate the adaptation and productivity of cup-plant across a range of plant densities (104,000 - 208,000 plants ha-1), cutting stages (early vegetative to seed formation), and different levels of N (0 - 400 kg N ha-1) and P (0 - 400 kg P2O5 ha-1) fertilization. Field experiments (40°S 73°W) were conducted over a 3-yr period in a subhumid, Mediterranean climate on three distinct andisols that originated from volcanic ash, with pH values of 5.2 to 5.6. The forage dry matter yield, plant components (stem count, stem diameter, plant height, and... |
| Tipo: Journal article |
Palavras-chave: Cup-plant; Cutting frequency; Fertilization; Forage crop; Nutritional quality; Population density. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100005 |
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| Velásquez,Alejandro; Pichard,Gastón. |
| Proteolytic activity of enzymatic extracts generated from rumen microorganisms cultivated in vitro was evaluated. The incubation of rumen fluid used different substrates to generate a higher enzyme concentration and promote a broad spectrum of hydrolytic activity. The composition of the substrates used in the cultivation of the fluid was enriched in protein, starch or cell wall. Enzyme preparations were evaluated by incubating in 30 mL of buffer 50 mM Tris-HCl (pH 6.5) at 39 °C during 48 hours, 100 mg of crude protein from feeds soybean meal, canola meal, sunflower meal, gluten feed, dehydrated alfalfa meal, berseem clover, oat forage and perennial ryegrass. Enzyme extracts from cultivated rumen fluid showed an average protein breakdown of 75.5%, in eight... |
| Tipo: Journal article |
Palavras-chave: In vitro rumen proteolysis; Ruminal enzymes; Streptomyces griseus; In situ method. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300005 |
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