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| Furian,TQ; Borges,KA; Pilatti,RM; Almeida,C; Nascimento,VP do; Salle,CTP; Moraes,HL de S. |
| The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular diagnosis; Non-serologic tests; Pasteurellosis; Serogroup. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200004 |
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| Pilatti,RM; Furian,TQ; Lima,DA; Finkler,F; Brito,BG; Salle,CTP; Moraes,HLS. |
| ABSTRACT Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fowl cholera; Inoculation; Pasteurella multocida; Pathogenicity index. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200255 |
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