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Production of cellulases by Penicillium oxalicum through solid state fermentation using agroindustrial substrates Biological Sciences
Bomtempo, Fabrícia Vieira Silva; Santin, Franciane Maria Mantovani; Pimenta, Raphael Sanzio; Oliveira, Deyla Paula de; Guarda, Emerson Adriano.
 The purpose of this study was to define the factors that influence the production of cellulases by Penicillium oxalicum, a strain obtained from a leaf-cutting ant colony and identified based on the ITS gene. The experimental design included solid state fermentation using sugarcane bagasse and lignocellulosic sorghum as the lignocellulosic substrate. The variables were analyzed using a 25-1 fractional factorial design, with three replicates on the central point. All the variables analyzed influenced the production of at least one of the three cellulose types analyzed. The highest values observed were: FPase 4.2 U g-1, CMCase 9.2 U g-1 and Avicelase 8.4 U g-1 using lignocellulosic sorghum as the substrate. The best conditions for enzyme production were:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sugarcane bagasse; Lignocellulosic sorghum; Cellulolytic enzymes. Microbiologia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32384
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Phytochemistry and antibacterial activity of aqueous and hydroalcoholic extracts of three medicinal plants against food pathogens Biological Sciences
Santos, Marcela Mona Sá; Silva, Filipe Miguel Pereira da; Silva, Juliana Fonseca Moreira da; Pimenta, Raphael Sanzio.
This study aimed to characterize the phytochemical compositions of three medicinal Brazilian plants’ leaves and bast extracts, and to determine their antibacterial activity on three foodborne and waterborne bacterial pathogens. Parkia platycephala, Pouteria ramiflora and Lophanthera lactescens leaves and basts were collected and aqueous and hydroalcoholic extracts were prepared. Qualitative screening of the phytochemical extracts was performed with three replicates and in triplicate in order to identify the bioactive compounds. The Minimal Inhibitory Concentration and Minimal Bactericide Concentration were determined by microdilution in broth and Escherichia coli, Salmonella typhimurium and Staphylococcus aureus growth was observed on agar plates....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catechins; Foodborne and waterborne pathogen; Minimal bactericide concentration; Minimal inhibitory concentration..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/39674
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