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	Provedor de dados BJM (1) Autor Chanitnun,Kankiya (1) Pinphanichakarn,Pairoh (1) Palavra-chave Agricultural residues (1) Glucose(xylose) isomerase (1) Production (1) Purification (1) Streptomyces (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2012 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Glucose(xylose) isomerase production by Streptomyces sp. CH7 grown on agricultural residues BJM Chanitnun,Kankiya; Pinphanichakarn,Pairoh. Streptomyces sp. CH7 was found to efficiently produce glucose(xylose) isomerase when grown on either xylan or agricultural residues. This strain produced a glucose(xylose) isomerase activity of roughly 1.8 U/mg of protein when it was grown in medium containing 1% xylose as a carbon source. Maximal enzymatic activities of about 5 and 3 U/mg were obtained when 1% xylan and 2.5% corn husks were used, respectively. The enzyme was purified from a mycelial extract to 16-fold purity with only two consecutive column chromatography steps using Macro-prep DEAE and Sephacryl-300, respectively. The approximate molecular weight of the purified enzyme is 170 kDa, and it has four identical subunits of 43.6 kDa as estimated by SDS-PAGE. Its Km values for glucose and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural residues; Glucose(xylose) isomerase; Production; Purification; Streptomyces. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300035 Registros recuperados: 1 Primeira ... 1 ... Última

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