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Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea BJM
Kaewklom,Siriporn; Samosornsuk,Seksun; Pipatsatitpong,Duangnate; Aunpad,Ratchaneewan.
Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic protein (cja). The partially purified bacteriocin (PPB) containing colicin type 7 of strain 35/44 was prepared and used for characterization. The antibacterial activity of PPB against a total of 17 selected Gram-positive and Gram-negative bacteria was tested. It was found that PPB of strain 35/44 was active against E. coli O157, S. sonnei and S. boydii. The sensitivity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella sonnei; Colicin type 7; Diarrhoea.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300010
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