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การใช้เซลล์แขวนลอยและสารสกัดหยาบของแบคทีเรีย Bacillus licheniformis BFP011 ในการควบคุมโรคแอนแทรคโนสบนผลพริก Thai Agricultural
Pattamawan Maneesuwan; Pisan Sirithorn.
The five concentrations of cell suspension of Bacillus licheniformis BFP011 each applied to reduce the disease symptom caused by Colletotrichum capsici using the detached fruit technique with Completely Randomized Design (CRD). The statistical analysis indicated that inoculation of cell suspension prior to inoculation of C. capsici gave more disease severity reduction than inoculation of C. capsici prior to inoculation of cell suspension. The optimal concentration that reduced disease severity; without adverse effect was at 1x104-1x105 cfu/ml prior to inoculation of C. capsici for 24 hours as disease severity was reduced by 35.8% and 34.8%, respectively. The cell suspension at the concentrations between 1x106 to 1x108 cfu/ml induced fruit rot to 10-50%....
Tipo: PhysicalObject Palavras-chave: Pepper; Anthracnose; Plant diseases; Fungi; Colletotrichum capsici; Fungus control; Plant disease control; Bacillus licheniformis; BFP011; Cell suspension; Crude extract; Disease severity; พริก; โรคแอนแทรคโนส; โรคพืช; เชื้อรา; การควบคุมโรค; จุลินทรีย์ปฏิปักษ์; เชื้อแบคทีเรีย; สารสกัดหยาบ; เซลล์แขวนลอย.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5795
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ผลของแบคทีเรีย Bacillus licheniformis BFP011 ในการยับยั้งเชื้อรา Colletotrichum capsici สาเหตุโรคแอนแทรคโนสของพริก Thai Agricultural
Pattamawan Maneesuwan; Pisan Sirithorn.
Capability of 11 isolates of Bacillus spp. previously reported as antagonistic against some plant pathogens was tested. This is to inhibit the growth of four isolates of Colletotrichum capsici isolated from stalk, leaf, fruit and 158 ci/KU, by employing Completely Randomized Design (CRD) consisting of 11 treatments with 4 replications. The results showed that B. licheniformis BFP011 exhibited the most promising inhibition. Similarly, the evaporated crude extract produced by B. licheniformis BFP011 was able to inhibit greatly growth of C. capsici. When mycelium and spores of C. capsici were immersed in cell suspension at 1x108 cfu/ml and the evaporated crude extract at 1,000 mg/ml of B. licheniformis BFP011 for 24 hours. It is obvious that abnormal hypha...
Tipo: PhysicalObject Palavras-chave: Pepper; Anthracnose; Plant diseases; Fungi; Colletotrichum capsici; Fungus control; Plant disease control; Bacillus licheniformis; BFP011; Cell suspension; Crude extract; Disease severity; พริก; โรคแอนแทรคโนส; โรคพืช; เชื้อรา; การควบคุมโรค; จุลินทรีย์ปฏิปักษ์; เชื้อแบคทีเรีย; สารสกัดหยาบ; เซลล์แขวนลอย; โรคกุ้งแห้ง.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5796
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ชีวภัณฑ์เชื้อแบคทีเรียปฏิปักษ์ Bacillus subtilis B076 เพื่อการเคลือบเมล็ดและพ่นทางใบ เพื่อควบคุมเชื้อแบคทีเรีย Acidovorax avenae subsp. citrulli Thai Agricultural
Kuson Thomma; Pisan Sirithorn.
Antagonistic activity of bacterium B. subtilis B076 on nutrient agar medium (NA) to Acidovorax avenae subsp. citrulli (Aac) isolate KK9 causal agent of bacterial fruit blotch, in the laboratory of Agricultural Biotechnology Research Center for Sustainable Economy Khon Kaen University. The results revealed that the B. subtilis B076 could exhibit the inhibition zone of diameter 1.94 cm. When cultured the bacterium antagonist in nutrient broth (NB) added with 2% glucose, the maximum ratio of heat tolerant cells production (61%) was obtained five days of incubation. The pellets of antagonistic bacteria were freeze dried by lyophilizer, the number of bacterial cell declined by 42.7%. The dust of bacterium after homogenizing with seed coating formulation...
Tipo: PhysicalObject Palavras-chave: Cantaloupe; Bacterial antagonist; Bacillus subtilis; B076; Bacterial fruit blotch; Seed coating; Seed treatment; Spraying; Biological control; Disease control; Acidovorax avenae; Formulations; แคนตาลูป; แบคทีเรียแบซิลลัส ซับทิลิส; เชื้อแบคทีเรียปฏิปักษ์; โรคผลเน่า; การเคลือบเมล็ด; การฉีดพ่นทางใบ; การยับยั้งการเจริญของเชื้อแบคทีเรีย; การควบคุมโรคโดยชีววิธี; ชีวภัณฑ์สูตรน้ำ; ประสิทธิภาพการควบคุมเชื้อแบคทีเรีย.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5736
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ผลของแบคทีเรียปฏิปักษ์ Bacillus subtilis B006 ในการเคลือบเมล็ด เพื่อควบคุมเชื้อรา Botryosphaeria rhodina สาเหตุโรคยางไหลของแตง Thai Agricultural
Kuson Thomma; Pisan Sirithorn.
Study was conducted to test find out the efficiency of the antagonistic bacterium Bacillus subtilis B006 against to Botryosphaeria rhodina cause of gummosis disease of cucurbit. The bacterium was determined its inhibition capability on the growth of B. rhodina 14 isolates. The results revealed that the antagonistic capability was significantly different into 9 groups. This bacterium was cultured in nutrient broth (NB) added 2% glucose and incubated at 28°C for 3, 5, 7 and 9 days and then, treated at 80°C for 30 min for heat tolerance cells. The maximum heat tolerant cells production (46-47%) was obtained 5-7 days of incubation. Hence the antagonistic bacterium B006 was cultured on such medium and temperature for 5 days and used to incorporate into four...
Tipo: Collection Palavras-chave: Antagonistic bacterium; Bacillus subtilis; Seed coating; Cucurbit; Botryosphaeria rhodina; Gummosis disease; แตง; แบคทีเรียปฏิปักษ์; แบซิลลัส ซับทิลิส; โรคยางไหล; การเคลือบเมล็ด; การควบคุมเชื้อรา่; การยับยั้งการเจริญของเชื้อรา; ต้นกล้า; เปอร์เซ็นต์การงอก.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5182
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