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| De Stefano,Eliana; Araújo,Wanderley Pereira de; Passos,Estevão de Camargo; Pituco,Edviges Maristela. |
| No ano de 2000 foram pesquisadas 1099 amostras de soro de bovinos de corte, adultos, pertencentes à seis rebanhos situados nos municípios de Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, região de Araçatuba. A técnica empregada para a dosagem de anticorpos para o vírus da Estomatite Vesicular foi a soroneutralização em microplacas, utilizando a cepa Indiana 1 Costa Rica/72 com título de 10(6,0) DICT50/50mL. As células utilizadas foram as da linhagem de Rim de Macaco Verde Africano (VERO-CCL 81), provenientes do American Type Cell Colection (ATCC). O título de anticorpos de cada amostra de soro, foi calculado como a recíproca da maior diluição do soro expresso em log10, que protegeu 100,00% da monocamada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Estomatite vesicular animal; Anticorpos; Bovinos de corte. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000100003 |
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| Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela. |
| ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600455 |
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| Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela. |
| ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of 100... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500451 |
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