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Brotas, V; Plantecuny, Mr. |
A HPLC protocol is described which permits analysis of the pigment composition of coastal and intertidal sediments. The HPLC technique has been extensively applied to phytoplankton pigments for fifteen years. Papers dealing with living benthic microphytic populations have been more rare, due to complexity existing in the sediment surface, where pigments (chlorophylls and carotenoids) from several taxonomic groups of microalgae are mixed with degraded pigments from senescent phytoplankton cells and macrophytic material. The present paper describes in detail a protocol adapted from Kraay et al. (1992), which allows a good discrimination of photosynthetic pigments of microphytobenthos communities. The following procedure is recommended. Sediment samples are... |
Tipo: Text |
Palavras-chave: Plants pigments; Sediments; Protocol; HPLC. |
Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00096/20730/18367.pdf |
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Barranguet, C; Alliot, E; Plantecuny, Mr. |
Microphytobenthos activity in coastal waters has been studied at two shellfish fanning sites: an oyster farm in Thau lagoon and a mussel farm in Carteau bay (NW Mediterranean coast). In both cases, shellfish are cultivated on ropes hanging from rigid structures (tables) in shallow waters (- 5 m). An ''under table'' station and an ''outside table'' (reference) station were chosen at each site. Oxygen and ammonia fluxes at the water-sediment interface were measured in dark and transparent benthic chambers. Chlorophyll a concentrations were two- to threefold higher in ''under table'' sediments than outside (260 mg Chl a m(-2) under the oyster table, 436 mg Chl a m(-2) under the mussel table in winter). Net oxygen fluxes were negative at all stations, except... |
Tipo: Text |
Palavras-chave: MICROPHYTOBENTHOS; FLUXES; PRIMARY PRODUCTION; SHELLFISH CULTIVATION. |
Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00098/20884/18498.pdf |
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Grenz, C; Plantecuny, Mr; Plante, R; Alliot, E; Baudinet, D; Berland, B. |
Benthic chambers were used to investigate fluctuations in nutrient fluxes and oxygen changes when comparing two benthic biota. Muddy sediments located under mussel-cultivation ropes were compared with a station outside the mussels' influence. Biodeposition from the mussels results in a drastic increase in the nutrient fluxes. A standard procedure is established in order to give reliable comparisons between stations and seasons: as a rule it should include six-hour periods of incubation with sampling intervals of two hours and at least four benthic chambers - preferably six - at each station. |
Tipo: Text |
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Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00102/21373/18973.pdf |
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