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การตรวจสอบความหลากหลายของสายพันธุ์เชื้อราสาเหตุโรคไหม้ของข้าวในประเทศไทย Thai Agricultural
Poonsak Mekwatanakarn; Payorm Cobelli; Acharaporn Na Lampang Noenplab; Thanomjit Rithmontree; Kulchana Ketsuwan; Chanasirin Klinmanee; Sanguan Teang Deerith.
Rice blast disease is the major disease in rainfed lowland area of Thailand. The causal fungus of this disease is Pyricularia grisea Sacc. Variation of this fungus was higher than other rice pathogens. This report used near isogenic lines (NILs) to detect the variation, pathothype and virulence spectrum of the fungus in Thailand during 2002 - 2005. Trap blast nurseries consisted of 9 Thai local cultivars and 18 NILs were conducted at Ubon Ratchathani Rice Research Center, Khon Kaen Rice Research Center, Phrae Rice Research Center, Phitsanulok Rice Research Center, and Phatthalung Rice Research Center. Single conidia was isolated from infected rice leaves. Virulence spectrum of blast populations were screened on 18 NILs. Two thousand four hundred seventy...
Tipo: Collection Palavras-chave: Rice; Blast disease; Pathotype; Near isogenic lines; Diversity; ข้าว; โรคไหม้; เชื้อสาเหตุ; สายพันธุ์เชื้อ; ยีนต้านทานเดี่ยว; ความหลากหลายของเชื้อ; เชื้อรา.
Ano: 2007 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5300
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ความก้าวหน้าการผนวกยีนความต้านทานโรคไหม้ ดินเค็ม และเพลี้ยกระโดดสีน้ำตาล ให้ข้าวขาวดอกมะลิ 105 โดยใช้เครื่องหมายโมเลกุล Thai Agricultural
Uraiwan Kotchasatit; Poonsak Mekwatanakarn; Jirapong Jairin; Duangjai Suriya-arunroj; Varapong Chamarerk; Anuchart Kotchasatit; Krissana Sattyakul.
Most farmers in the Northeastern Thailand grow Khao Dawk Mali 105, a non-glutinous variety because of its premium cooking and eating quality and its aroma. However, KDML105 is susceptible to blast, brown planthopper and soil salinity. In order to maintain its yield stability, it is necessary to incorporate those above mentioned traits into KDML105 background. By using molecular markers to select for those plants carrying the target genes in each generation, it will help improving the selection efficiency in term of accuracy and time consumed. The experiment was conducted at Ubon Ratchathani Rice Research Center during 2007-2012 using the lines derived from the backcrossing program of KDML105; UBN03004 (IR77955-14-17*2/B.T) and UBN03007...
Tipo: PhysicalObject Palavras-chave: Khao Dawk Mali 105; Glutinous rice; Blast resistance; Brown planthopper resistance; Salt tolerance; ข้าวเจ้า; พันธุ์ขาวดอกมะลิ 105; โรคไหม้; เพลี้ยกระโดดสีน้ำตาล; ดินเค็ม; การถ่ายทอดยีน; ความต้านทานต่อโรค; ความต้านทานต่อแมลง; ความทนทานดินเค็ม.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5598
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การจัดทำลายพิมพ์เอกลักษณ์ดีเอ็นเอข้าวพื้นเมืองไทยที่เก็บอยู่ในธนาคารเชื้อพันธุ์ข้าวแห่งชาติ Thai Agricultural
Payorm Cobelli; Varapong Chamarerk; Poonsak Mekwatanakarn; Phikul Leelagud; Somsong Chotechuen; Sommai Seewisut.
SSR molecular markers SSR are tandem arranged repeats of short DNA motifs (1-6 bp in length) distributed throughout the genome that frequently exhibit variation in the number of repeats at the locus of each rice variety. The SSR markers, therefore, are suitable for detecting polymorphisms between closely related genotypes of Thai local rice varieties from the National Rice Germplasm Bank. In this research, DNA fingerprinting was conducted using 8 Thai local rice varieties. i.e., Niaw Sanpahtawng (NSPT_GB), Khao Gaw Diaw (KGD_GB), Niaw Dam (ND_GB), Khao tah Haeng 17 (KTH17_GB), Jao’ Haw (JH_GB), Pin Gaew 56 (PG56_GB), Jek Chuey Gahb Khiaw (JCGK_GB), Sang Yod Pattalung (SYPL_GB), and 5 standard varieties, i.e., Khao Dawk Mali 105 (KDML105_GB, KDML105_C),...
Tipo: PhysicalObject Palavras-chave: Thai local rice; DNA fingerprinting; Molecular markers; National Rice Germplasm Bank; ข้าวพื้นเมืองไทย; ลายพิมพ์เอกลักษณ์ดีเอ็นเอ; เครื่องหมายโมเลกุล; การเรียงตัวของดีเอ็นเอ; ความใกล้ชิดทางพันธุกรรม; พันธุ์.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5225
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การตรวจวิเคราะห์ข้าวและสินค้าข้าวดัดแปรพันธุกรรมด้วยปฏิกิริยาลูกโซ่ ในสภาพเวลาจริง Thai Agricultural
Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn.
Genetically modified (GM) insecticidal rice line, Bt63 (Shanyou63), has been developed in China and found unauthorized in processed rice products in many countries. This transgenic rice contains a copy of the synthetic DNA sequence with two genes: CryIA(b) and CryIA(c). These genes encode for the respective Bt toxins which is lethal to Lepidoptera. The aim of this study was to determine the contamination of Bt63 in non-labelled commercially available processed rice products from supermarkets. In this study, a real-time Polymerase Chain Reaction (PCR) was used to simultaneously determine the contamination of Bt63. The survey was conducted on a total of 21 and 42 non-labeled samples of processed rice products collected in the years 2011 and 2012,...
Tipo: Collection Palavras-chave: GM rice; Bt63; Real-time PCR; ข้าวดัดแปรพันธุกรรม; Bt63; ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง.
Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5923
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การตรวจวิเคราะห์สินค้าข้าวดัดแปรงพันธุกรรมด้วยปฏิกิริยาลูกโซ่ในสภาพเวลาจริง Thai Agricultural
Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn.
Currently, there are several reports on GM rice illegally available on market. To set up a defense measure in order to prevent GM contamination in Thai rice market, this activity was conducted by analyzing for CaMV 35S Promoter, NOS terminator and a transgene CryIA(b), which were carrying by the Bt63 rice. The Bt63 rice is an insect-resistant rice variety developed by the People Republic of China. The analyses were done by using a Real-Time PCR technique. DNA samples were extracted from rice products using a GeneScan extraction method and confirmed by a PCR using a primer pair KVM159/KVM160, which amplifies a rice species specific PLD gene encoding for an enzyme Phospholipase D. It was found that all samples could be amplified showing a PLD gene with a...
Tipo: Collection Palavras-chave: GM rice; Bt63 rice; Real-time PCR; ข้าวดัดแปรพันธุกรรม; ข้าว Bt63; ปฏิกิริยาลูกโซ่แบบในสภาพเวลาจริง.
Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5850
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