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| Huvet, Arnaud; Daniel, Jean-yves; Quere, Claudie; Dubois, Solene; Prudence, Marie; Van Wormhoudt, A; Sellos, D; Samain, Jean-francois; Moal, Jeanne. |
| A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed at different levels depending on tissue type. A strong preferential expression of both amylase genes was observed in the digestive gland leading to high amylase activities, in accordance with the digestive function of this organ. Traces of gene expression and activity were observed in other analysed tissues. These appeared higher in labial palps than in other... |
| Tipo: Text |
Palavras-chave: Oyster; Gene expression; Food regulation; Digestive enzyme activity; Alpha amylase. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-394.pdf |
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| Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud. |
| This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,... |
| Tipo: Text |
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf |
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| Prudence, Marie. |
| Two amylase genes, A and B, from the oyster Crassostrea gigas were characterized. Using PCR-RFLP, 6 and 4 alleles, respectively, were described for the amylase genes A and B. The roles of A and B amylase genes were investigated experimentally. They are expressed during larval and adult stages, and A transcripts are more abundant than B. The A transcript increases significantly with temperature, in high trophic conditions. However, A and B transcript levels do not change when food quantity increases although amylase activity augments. The level of B is correlated with dietary starch quantities, whereas the amount of A appears to remain constant ; simultaneously amylase activity decreases and the KM increases. These results suggest that expression of B is... |
| Tipo: Text |
Palavras-chave: Enzymatic analyse; Genetic polymorphism; Genes; Nutrition; Growth; Environmental effects; Oysters; Amylases; Amylases; Analyse enzymatique; Polymorphisme génétique; Gènes; Nutrition; Croissance; Effets de l'environnement; Huîtres; Amylases; Amylases. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/these-1700.pdf |
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