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Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family Electron. J. Biotechnol.
Rampadarath,Sillma; Puchooa,Daneshwar; Bal,Subhasisa.
Background The classification of diversity in germplasm collections is important for plant breeding. The repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX primers. Results The agglomerative cluster analysis was used to evaluate the scoring data. BOX and REP gave amplification with polymorphism of 98.84% and 100% respectively. REP marker demarcated between the subgenus peltatae. Both markers confirmed Jatropha tanjorensis as a natural hybrid between Jatropha gossypifolia and Jatropha curcas. Five random sequences from the rep-PCR gels were chosen, cloned and sequenced. The blast results demonstrated that the amplified...
Tipo: Journal article Palavras-chave: Euphorbiaceae; Genetic diversity; Jatropha; Mitochondrial genome; Rep-PCR.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600005
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Biotechnology in Mauritius: current status and constraints Electron. J. Biotechnol.
Puchooa,Daneshwar.
Agriculture today faces the challenge of sustainable productivity and improved food security on a shrinking area of land under cultivation. Until recently, biotechnology has been viewed as an expensive technology affordable only by rich industrial countries. However, it is now increasingly considered as an essential tool for developing countries to tackle the numerous problems of underdevelopment. Unfortunately, to date, Mauritius, like many other countries in Africa, lags far behind in development, use and commercialisation of biotechnology. This paper outlines the current status of biotechnology in Mauritius, some important constraints faced, as well as how policy makers are planning to make Mauritius “assume the role of a service provider and...
Tipo: Journal article
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200004
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