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XU,Li-na; LU,Bi-nan; HU,Ming-ming; XU,You-wei; HAN,Xu; QI,Yan; PENG,Jin-yong. |
Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Cell cycle arrest; Death receptor pathway; Mitochondrial pathway; Natural product. |
Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300003 |
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