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	Provedor de dados Neotropical Entomology (2) Autor QUERINO,RANYSE B. (2) ZUCCHI,ROBERTO A. (2) CIOCIOLA JR.,AMÉRICO I. (1) MORAES,REGINA C.B. DE (1) STOUTHAMER,RICHARD (1) Palavra-chave Insecta (2) Biological control (1) Egg parasitoids (1) Geometric morphometrics (1) ITS2 region (1) Parasitoids (1) RDNA (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2002 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Molecular Tool for Identification of Closely Related Species of Trichogramma (Hymenoptera: Trichogrammatidae): T. rojasi Nagaraja & Nagarkatti and T. lasallei Pinto Neotropical Entomology CIOCIOLA JR.,AMÉRICO I.; QUERINO,RANYSE B.; ZUCCHI,ROBERTO A.; STOUTHAMER,RICHARD. The sequence of the ITS2 (internally transcribed spacer 2) region of the rDNA was used to identify two closely related Trichogramma species: T. rojasi and T. lasallei, the second species recently reported in Brazil. The DNA of the samples was extracted using Chelex 100. The PCR product was linked to a pGEM-T® vector and both were then placed into Petri dishes to grow white colonies, which demonstrate the correct ligation between the vector and the DNA. From these colonies, 10µl were purified and sequenced using a special kit, and the sequences aligned using the ESEE 3.0 software. Specific primers were efficient to amplify the ITS2 rDNA region of the Brazilian Trichogramma. The sequence of each species differed in length and in nucleotide position, showing... Tipo: Info:eu-repo/semantics/other Palavras-chave: Insecta; ITS2 region; Biological control; RDNA; Parasitoids. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000400010 Relative warp analysis to study morphological variations in the genital capsule of Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) Neotropical Entomology QUERINO,RANYSE B.; MORAES,REGINA C.B. DE; ZUCCHI,ROBERTO A.. The influence of the host on the morphology of the male genital capsule of Trichogramma pretiosum Riley was studied by geometric morphometric analysis. Specimens of the parasitoid obtained from 10 different Lepidoptera host species were used. Initially, landmarks were defined and Cartesian coordinates (x and y) for each landmark of the genital capsule of all specimens were obtained. The anatomic landmarks transformed to Cartesian coordinates were then analyzed by generalized orthogonal least-square procedure. Consensus configuration of the genital capsule for all specimens was obtained and partial and relative warps were determined. Fifteen landmarks generated 24 relative warps for each specimen. The ordination of the relative warps (alpha = 0), using... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Geometric morphometrics; Egg parasitoids. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000200007 Registros recuperados: 2 Primeira ... 1 ... Última

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