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RestrictionDigest: A powerful Perl module for simulating genomic restriction digests Electron. J. Biotechnol.
Wang,Jinpeng; Li,Li; Qi,Haigang; Du,Xuedi; Zhang,Guofan.
Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction enzymes. A key factor of this method is to determine the restriction enzyme(s). But there are few computer programs which can evaluate the usability of restriction enzymes in reduced-representation sequencing. SimRAD is an R package which can simulate the digestion of DNA sequence by restriction enzymes and return enzyme loci number as well as fragment number. But for linkage mapping analysis, enzyme loci distribution is also an important factor to evaluate the enzyme. For phylogenetic studies, comparison of...
Tipo: Journal article Palavras-chave: Genotyping; Reduced-representation libraries; Reduced-representation sequencing.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300003
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