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| Hongming,Liu; Xu,Lou; Zhaojian,Ge; Fan,Yang; Dingbin,Chen; Jianchun,Zhu; Jianhong,Xu; Shunpeng,Li; Qing,Hong. |
| The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L−1 FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl >... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fenoxaprop-P-ethyl; Microbial degradation; Rhodococcus ruber JPL-2; FE -hydrolyzing carboxylesterase gene. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200425 |
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