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Gonad volume assessment in the oyster Crassostrea gigas: Comparison between a histological method and a Magnetic Resonance Imaging (MRI) method ArchiMer
Flahauw, Emilie; Quellec, Stephane; Davenel, Armel; Degremont, Lionel; Lapegue, Sylvie; Hatt, Philippe-jacques.
Previous studies comparing Crassostrea gigas selected lines with different susceptibility to summer mortality concluded that the higher the reproductive effort the lower the survival. But theywere lacking individual followup of this reproductive effort which would be of great interest to help us to understand the physiology of the oysters facing summermortalities. Themost frequently usedmethod for assessing the volume of the gonad is to measure the area it occupies on histological sections. Gonad measurement by magnetic resonance imaging, in contrast, is non-destructive and therefore makes it possible to assess individual evolution as well as to compare individuals. The present study compared the values of gonad volume by thismethodwith the values of gonad...
Tipo: Text Palavras-chave: Oyster; Crassostrea gigas; Reproduction; Histology; Magnetic resonance imaging; MRI.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00102/21309/19410.pdf
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Magnetic resonance imaging as a means to assess the body growth and the gonad development of the oyster Crassostrea gigas ArchiMer
Hatt, Philippe-jacques; Davenel, Armel; Eliat, Pierre-antoine; Quellec, Stephane.
To make a preliminary exploration of the possibilities and limits of magnetic resonance imaging (MRI) for assessing body growth and gonad development of the Pacific oyster, Crassostrea gigas, individual monitoring was conducted on diploid and triploid oysters in their second year, maintained from February to October in a pond on the French Atlantic coast. Magnetic resonance imaging was done on each oyster on five dates during the study period. From these images, variations in the volumes of the flesh, digestive gland-gonad and adductor muscle, and the surface of the gills were measured over time. Apart from the well known differences between diploids and triploids related to maturation of gametes, other original observations of assessing growth were made...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Gonad development; Growth; Magnetic resonance imaging; MRI.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6872.pdf
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Individual monitoring of gonad development in the European flat oyster Ostrea edulis by in vivo magnetic resonance imaging ArchiMer
Davenel, Armel; Gonzalez, Ricardo; Suquet, Marc; Quellec, Stephane; Robert, Rene.
Previous studies have shown that magnetic resonance imaging is a very appropriate non-invasive technique for quantifying the growth of somatic and gonadic tissues and sex determination in the Pacific oyster, Crassostrea gigas. Despite a thinner gonad, harder to distinguish than that of the Pacific oyster, we showed in this study that it was possible to distinguish and quantify the development of the gonad of the European flat oyster, Ostrea edulis, using a 3D MRI sequence with a suitable finer resolution. Compared to T2-weighted images, theoretically the most appropriate for good anatomical description, T1-weighted images were more suited to gonad observation. The gonad development was quantified by the number of voxels with higher grey level. Larvae were...
Tipo: Text Palavras-chave: Oyster; Ostrea edulis; Gonad development; Magnetic resonance imaging.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12508/9363.pdf
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