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	Provedor de dados BABT (1) Autor Araújo,Elza Fernandes de (1) Chaves-Alves,Virgínia Maria (1) Coelho,Jorge Luiz Cavalcante (1) Querido,André Luiz de Souza (1) Palavra-chave Partial purification (1) Penicillium expansum (1) Xylan/xylanases (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2006 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Partial purification and characterization of xylanase produced by Penicillium expansum BABT Querido,André Luiz de Souza; Coelho,Jorge Luiz Cavalcante; Araújo,Elza Fernandes de; Chaves-Alves,Virgínia Maria. An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mumol min-1 mug -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+. Tipo: Info:eu-repo/semantics/article Palavras-chave: Partial purification; Xylan/xylanases; Penicillium expansum. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400016 Registros recuperados: 1 Primeira ... 1 ... Última

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