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Investigating the relationship between embryotoxic and genotoxic effects of benzo[alpha]pyrene, 17 alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos ArchiMer
Wessel, Nathalie; Rousseau, Sabrina; Caisey, Xavier; Quiniou, Francoise; Akcha, Farida.
Genotoxicity biomarkers are widely measured in ecotoxicology as molecular toxic endpoints of major environmental pollutants. However, the long-term consequences of such damage still have to be elucidated. Some authors have suggested that the accumulation of unrepaired DNA lesions could explain the embryotoxicity of certain chemical pollutants. As embryotoxicity exerts a direct impact on the recruitment rate, genotoxicity could be closely related to disturbances of ecological concern and produce a possible impact upon population dynamics. The aim of the present work was to study the genotoxicity and the embryotoxicity of three relevant pollutants for oyster embryos: the polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP), the synthetic estrogenic...
Tipo: Text Palavras-chave: 17 alpha ethinylestradiol; BaP; Endosulfan; DNA damage; Embryotoxicity; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4058.pdf
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Utilisation des bioessais sur les embryons de bivalves dans le cadre d'une surveillance des effets biologiques ArchiMer
Quiniou, Francoise; Toularastel, France; Le Fevre-lehoerff, Geneviève.
La surveillance d'un milieu peut se faire par la mesure des concentrations de "contaminants" dans l'eau, la matière vivante et le sédiment. C'est d'ailleurs ce qui est réalisé par le RNO sur le littoral français depuis 1974. Le dosage de ces contaminants donne une évaluation des niveaux de leurs concentrations dans les zones étudiées. Un effet de l'accumulation des contaminants dans la matière vivante peut aussi être mesuré grâce aux techniques biochimiques du type mesure de l'Acetylcholinestérase, EROD (GALGANI, 1987). La mesure des effets biologiques sur les organismes marins peut être réalisée par le biais des bioessais. Ces derniers permettent d'évaluer la qualité biologique d'un milieu : eau, sédiment ou rejets et émissaires divers. (Les réponses des...
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/00132/24360/22360.pdf
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Effect of Bentazon on Growth and Physiological Responses of Marine Diatom: Chaetoceros gracilis ArchiMer
Hourmant, Annick; Amara, Anis; Pouline, Patrick; Durand, Gael; Arzul, Genevieve; Quiniou, Francoise.
The herbicide bentazon (CASRN 25057-89-0) is extensively used in agriculture in Brittany (France) to replace atrazine. Bentazon is not readily adsorbed by soil and therefore it enters adjacent freshwater ecosystems, making its way to estuarine and marine waters areas. Information regarding its effects on marine ecosystems is scarce. Phytotoxicity assessments were conducted in the laboratory on the common diatom Chaetoceros gracilis using both the active ingredient and its formulated product (Basamais). The 3 day EC50 Using cell counts were, respectively, 150 mg/L and 60 mu g/L for bentazon active ingredient and for bentazon-formulated while cellular volume was increased. Although bentazon is known as a photosystem II inhibitor, it produced an increase of...
Tipo: Text Palavras-chave: Photosynthesis; Marine diatom; Growth; Chlorophylls; Chaetoceros gracilis; Carotenoids; Bentazon; ATP.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6637.pdf
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Evaluation of biomarkers in oyster larvae in natural and polluted conditions ArchiMer
Damiens, Gautier; His, Edouard; Gnassia Barelli, Mauricette; Quiniou, Francoise; Romeo, Michèle.
Crassostrea gigas D-shaped larvae were subjected to different conditions of temperature and salinity for 24 h and four biomarkers (acetylcholinesterase (AChE) activity, thiobarbituric acid reactive substances (TBARS) levels, glutathione S-transferase (GST) and catalase (CAT) activities) were measured. AChE activity decreased when salinity increased from 25 to 30 and 35 psu at 20 and 25 degreesC. Temperature did not seem to have an influence on AChE activity. TBARS levels increased as a function of salinity when the temperature was maintained at 20 degreesC, whereas at 25 degreesC no effect of salinity could be observed. Variations in GST and CAT activities were not significant with salinity and temperature except that catalase activity was higher at 25...
Tipo: Text Palavras-chave: Malathion; Carbofuran; Abiotic factors; Crassostrea gigas; Mollusc larvae; Biomarker.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-686.pdf
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Taux de réussite du développement embryonnaire de la moule et de l'huitre creuse en présence d'eau ou de sédiment cotier de la baie de Seine ArchiMer
Quiniou, Francoise.
Une étude préalable à la mise en route, dans le cadre du RNO, d'un programme de surveillance des effets biologiques a été menée en recherchant l'effet de la présence d'eau ou de sédiment côtier de la baie de Seine sur le développement embryonnaire de la moule et de l'huître creuse. Les résultats montrent que ce test est très intéressant à proposer dans le cadre d'une surveillance biologique, après mise au point spécifique du protocole expérimental pour le suivi du sédiment.
Tipo: Text Palavras-chave: Surveillance biologique; Baie de Seine; Développement embryonnaire; Moule; Huître creuse.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00132/24352/22351.pdf
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Biodragages - Protocole de suivi de l'efficacité et de l'impact potentiel ArchiMer
Alzieu, Claude; Andral, Bruno; Bassoulet, Philippe; Gourmelon, Michele; Le Quillec, Régis; L'Yavanc, Jacky; Quiniou, Francoise.
Les traitements in situ des sédiments, ou biodragages, consistent à disperser à la surface ou à injecter dans le sédiment des produits pulvérulents sur lesquels sont fixées des bactéries capables de dégrader les matières organiques. Peu de données expérimentales sont disponibles quant à l'efficacité et à l'impact de cette pratique qui, bien qu'utilisée dans certains milieux d'eau douce, n'a pas fait l'objet de réelles applications en milieu marin. L'offre de service de quelques opérateurs et la demande des gestionnaires des ports, ne peuvent être prises en considération, faute d'une connaissance approfondie des avantages et inconvénients de telles techniques. Il nous est donc apparu nécessaire de définir et de proposer, aux Services en charge des...
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Ano: 1999 URL: http://archimer.ifremer.fr/doc/00040/15121/12457.pdf
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Tests de toxicité : effets létaux et sublétaux. Synthèse bibliographique. ArchiMer
Quiniou, Francoise; Judas, Antoine.
Cette synthèse bibliographique, non exhaustive, a été réalisée dans le cadre du projet "rade de Brest" grâce à la collaboration d'Antoine JUDAS, contractuel de la CUB en 1995 (premier trimestre). Elle fait partie du projet "Critères physiologiques de la qualité du milieu" réalisé au laboratoire d'Ecotoxicologie de la Direction de l'Environnement Littoral de Brest. Ce document se présente sous la forme de listes dans lesquelles sont reportés les effets létaux et sublétaux de substances chimiques. Pour chaque substance sont indiqués: la forme chimique de la substance, l'espèce testée, le type d'essai réalisé, les concentrations entraînant 50% de létalité (LC50) ou 50% d'effet sublétal (CE50) ainsi que la référence des publications d'où proviennent les valeurs.
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Ano: 1995 URL: http://archimer.ifremer.fr/doc/00076/18759/16329.pdf
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Evaluation des risques liés à l'immersion des boues de dragage des ports maritimes. Colloque de restitution des résultats du programme PNETOX, Paris 3 et 4 octobre 2002 ArchiMer
Alzieu, Claude; Bocquene, Gilles; Delesmont, Régis; Dhainaut-courtois, Nicole; Empis, Anne; Forget, Joelle; Glemarec, Michel; Pavillon, Jean-françois; Pruvot, Chrystèle; Quiniou, Francoise.
RAPPEL DES OBJECTIFS - Les objectifs visés par l'étude à l'origine du présent contrat consistaient à proposer et réaliser une démarche d'analyse prévisionnelle des risques environnementaux applicable aux boues de dragages des ports maritimes et à leur immersion. L'objectif final était de fournir aux services portuaires et aux Cellules de la Qualité des Eaux Littorales du Ministère de l'Aménagement du Territoire et de l'Environnement, une méthodologie de prévision des risques environnementaux des immersions des matériaux de dragage, facilitant ainsi leur gestion.
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Ano: 2003 URL: http://archimer.ifremer.fr/doc/00075/18613/16166.pdf
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In-situ microcosms, a tool for assessment of pesticide impacts on oyster spat (Crassostrea gigas) ArchiMer
Stachowski Haberkorn, S; Quiniou, Francoise; Nedelec, M; Robert, Rene; Limon, G; De La Broise, D.
Effects of the herbicide Basamais (bentazon) and the fungicide Opus (epoxiconazole) on oyster spat (Crassostrea gigas) were assessed using in-situ microcosms in a field experiment lasting 13 days. Six-week-old hatchery spat (mean size 1.1 mm), previously collected on PVC plates, was immersed in glass bottles filled with 200 mu m filtered seawater. Bottles were maintained underwater at 6 m depth and their water content changed every other day. Growth, measured as shell area index increase, was 126 +/- 4% in the control bottles. While no growth differences were observed between control and individual pesticide treatments at 10 mu g l(-1), oysters treated with a mix of 10 mu g l(-1) Opus and 10 mu g l(-1) Basamais showed a 50% growth reduction compared with...
Tipo: Text Palavras-chave: Epoxiconazole; Bentazon; Pesticide; Toxicity; Oyster; Microcosm.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4134.pdf
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Estimation de la qualité biologique de l'eau sur le littoral atlantique du Sud-Ouest de la France à l'aide des tests de croissance des larves de Mytilus edulis ArchiMer
His, Edouard; Quiniou, Francoise.
The quality of the seawater off the southwestern Atlantic coast of France was assessed by studying the effects of the water from three sites (Arcachon Bay, Mimizan and Capbreton) on larvae of the mussel (Mytilus edulis). Mussel larvae were raised in water samples taken at all three sites on March 12, 1991 and larval growth was monitored during 10 days. The results indicate that the water quality was best in the Bay of Arcachon, followed by Mimizan, whereas the water off Capbreton was possibly contaminated by pollutants.
Tipo: Text Palavras-chave: Croissance; Qualité de l'eau; Développement larval; Pollution; Mytilus edulis; Growth; Water quality control; Larval development; Pollution; Mytilus edulis.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00038/14903/12229.pdf
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Marine water quality assessment using transplanted oyster larvae ArchiMer
Quiniou, Francoise; Damiens, G; Gnassia Barelli, M; Geffard, A; Mouneyrac, C; Budzinski, H; Romeo, Michèle.
Active bio-monitoring in terms of biornarkers was attempted using Crassostrea gigas larvae produced in the laboratory and transplanted using special containers to two sites at the entrance (A) and inner part (P) of the harbour of Arcachon (French Atlantic Coast). The larvae were kept in the medium for 48 h. Their physiological status and their biomarker levels : acetylcholinesterase AChE, catalase CAT and glutathione S-transferase GST activities were determined together with metallothionein Wand Thiobarbituric Acid Reactive Substances TBARS concentrations. Copper and PAH (polycyclic aromatic hydrocarbon) concentrations were determined in the exposed larvae and in the sediments collected under the containers. Cadmium, lead and zinc could be also analyzed in...
Tipo: Text Palavras-chave: PAHs; Trace metals; Biomarkers; Active bio monitoring; Crassostrea gigas larvae.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2269.pdf
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Etude l'écotoxicité des sédiments dragues - Documents de synthèse et annexes - Projet du groupe GEODE ArchiMer
Quiniou, Francoise; Blanchard, Michel; Bocquene, Gilles; Burgeot, Thierry; Giboire, Louis; His, Edouard; Le Du, Anne.
La présente étude a pour objectif de sélectionner des méthodes de laboratoire et de terrain les mieux appropriées pour l'évaluation de l'écotoxicité de sédiments dragués : indicateurs biochimiques d'exposition (EROD et AChE), tests de toxicité sublétale (micro-organismes, algues, embryons de bivalve et extrait enzymatiques de poisson), indices physiologiques de bivalve (respiration, excrétion, mouvements valvaires). Une synthèse des résultats est présentée accompagnée, en annexes, des rapports des différents participants à l'étude.
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00104/21573/19153.pdf
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The Paracentrotus lividus populations from the northern Moroccan Atlantic coast: growth, reproduction and health condition ArchiMer
Bayed, Abdellatif; Quiniou, Francoise; Benrha, Ali; Guillou, Monique.
The condition of three intertidal Paracentrotus lividus populations, which inhabit an area between Rabat and Casablanca (Morocco) known by its high wave energy and its substantial anthropogenic disturbances were described. The growth rate of the juvenile sea urchins in this intertidal zone was low compared with more sheltered populations. A sharp increase in the gonad index was observed between January and March at the beginning of a period of algal production, and was concomitant with increases in seawater temperature and food consumption rate. This gonad index increase was followed by a single spawning period occurring between March and June, probably triggered by the phytoplankton bloom induced by upwelling conditions. Larval development in all three...
Tipo: Text Palavras-chave: Moroccan Atlantic; Growth; Population; Juvenile; Reproduction.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-602.pdf
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In vitro test-based comparison of pesticide-induced sensitivity in marine and freshwater phytoplankton ArchiMer
Arzul, Genevieve; Quiniou, Francoise; Carrie, Cecile.
This study aims to assess the effects of two pesticides, namely the insecticide carbofuran and the herbicide isoproturon, on monospecifically cultivated marine and freshwater phytoplankton according to standard methods. In the presence of pesticide, growth rates were lower in marine species Chaetoceros gracilis and Phaeodactylum tricornutum than in freshwater species Chlorella vulgaris and Selenastrum capricornutum after 72 hours. The EC50 values were obtained with the REGTOX Macro software, and the NEC values by applying the DEBtox model.
Tipo: Text Palavras-chave: REGTOX Macro; Pesticides; Marine phytoplankton; Freshwater phytoplankton; DEBtox model.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6605.pdf
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Testing methods to assess both the efficacy and ecotoxicity of antifouling coatings ArchiMer
Quiniou, Francoise; Compere, Chantal; Caisey, Xavier; Davy, Romain; Matarere, Marcel; Mazeas, Florence; Peleau, Michel.
Since 1998, the European Biocidal Products Directive (BPD; 98/8/EC), applied to 23 product types (PT) for specific uses, including PT 21 the « antifouling products », regulates biocidal products before they are placed on the market. On the one hand, the efficacy of active substances and their formulations must be demonstrated for their specific employment ; on the other hand their non impact on workers neither users and environment must be proved and specifically against non target species. Standard protocols for the assessment of efficacy or ecotoxicity of antifouling substances are proposed within the framework of the Ecopaint and Paintclean projects which are founded by the french industry Ministery and labellised by the “Pôle Mer” (Paca and...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00040/15133/12471.pdf
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Toxicity assessment of peptaibols and contaminated sediments on Crassostrea gigas embryos ArchiMer
Poirier, Laurence; Quiniou, Francoise; Ruiz, Nicolas; Montagu, Monique; Amiard, Jean-claude; Pouchus, Yves François.
Peptaibols are known membrane-modifying peptides that were recently detected in marine sediments and mussels collected from a shellfish farming area (Fier d'Ars, Atlantic coast, France). In this investigation, embryotoxicity bioassays with oysters (Crassostrea gigas) were performed to assess acute toxicity of alamethicin and different groups of peptaibols produced by a Trichoderma longibrachiatum strain isolated from marine environment. C gigas embryos appeared very sensitive to all the metabolites examined with higher toxic effects for long-sequence peptides (EC50 ranging from 10 to 64nM). D-shaped larvae with mantle abnormality were particularly noticed when peptaibol concentrations increased. Disturbances of embryogenesis were also observed following...
Tipo: Text Palavras-chave: Risk assessment; Embryotoxicity; Bivalve bioassay; Mycotoxins; Marine fungi.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2680.pdf
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Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry ArchiMer
Poirier, Laurence; Amiard, Jean-claude; Mondeguer, Florence; Quiniou, Francoise; Ruiz, Nicolas; Pouchus, Yves Francois; Montagu, Monique.
Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.
Tipo: Text Palavras-chave: Matched calibration; Matrix; Electrospray ionization ion trap mass spectrometry ESI IT MS; Marine fungal contamination; Trichoderma sp.; Fungal peptide metabolites.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2990.pdf
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Surveillance écologique et halieutique de l'environnement marin du site de la centrale de Penly (Manche Est) : Année 1990 ArchiMer
Arzul, Geneviève; Erard-le Denn, Evelyne; Halgand, Daniel; Quiniou, Francoise; Tetard, Alain; Toularastel, France; Vincke, Marie-claude.
Surveillance n°1, 2nd phase, of PENLY nuclear power-plant takes In account studies between April and September 1990. Hydrology, microbiology, phytoplankton, zooplankton, fishing are studied.
Tipo: Text Palavras-chave: Pelagos; Halieutique; Centrale nucléaire; Surveillance; Pelagos; FIshing; Nuclear power plant; Monitoring.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/00432/54392/55722.pdf
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Caractérisation des facteurs de risques associés au mortalités estivales - Synthèse du thème 3 - Risque associé au stress environnemental - 2002-2005 ArchiMer
Burgeot, Thierry; Gagnaire, Beatrice; Renault, Tristan; Haure, Joel; Moraga, D.; David, E.; Boutet, L.; Sauriau, P.g.; Malet, Nathalie; Bouchet, V.; Lapegue, Sylvie; Bouilly, Karine; Le Moullac, Gilles; Arzul, Genevieve; Knoery, Joel; Quiniou, Francoise; Bacher, Cedric; Soletchnik, Patrick.
Les mortalités d'huîtres observées sur les côtes françaises apparaissent principalement à proximité du sédiment et pendant la gamétogenèse. Les interactions de facteurs environnementaux sur les écosystèmes conchylicoles comme la température, les apports des bassins versants et le compartiment sédimentaire illustrent un risque de mortalité lié à différentes sources de stress (Thème 1). Les variations de la qualité de la ressource trophique pendant l'effort de gamétogenèse favorise une baisse de l'énergie disponible (Thème 2) et contraint l'organisme fragilisé à fournir un effort d'adaptation (Fent 2004). L'apparition d'un stress pendant cette période sensible des huîtres pourrait constituer un facteur aggravant de mortalité. L'objectif de cette étude est...
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Ano: 2006 URL: http://archimer.ifremer.fr/doc/00304/41538/40746.pdf
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Marine Sediment Contamination and Toxicity in Izmir Bay and Mytilene Harbor (Aegean Sea) ArchiMer
Oral, Rahime; Kostopoulou, Maria; Guida, Marco; Nikolaou, Anastasia; Quiniou, Francoise; Trifuoggi, Marco; Borriello, Ilaria; Vagi, Maria; D'Ambra, Antonello; Pagano, Giovanni.
The aim of the present study was to evaluate marine contamination and toxicity in sediment and pore water from the areas of Izmir Bay (Turkey) and Mytilene Harbor (Greece). The evaluation was performed in terms of selected toxic-metals, polycyclic aromatic compounds (PAR) and toxicity to sea urchin (Paracentrotus lividus) early development and fertilization. Significantly higher levels of metals and total PAR were detected in Izmir vs. Mytilene sediment, and the highest contamination was detected in Izmir Bay innermost sites. Bioassays were carried out in P. lividus embryos and sperm, by evaluating developmental defects and changes in fertilization success by exposing sea urchin embryos or sperm to sediment/seawater suspensions. Whole sediment (WS) showed...
Tipo: Text Palavras-chave: Sediment toxicity; Whole sediment; Pore water; Sea urchins; Aegean Sea.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00125/23600/21447.pdf
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