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Registros recuperados: 5
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In vitro Propagation of Cedar (Cedrela odorata L.) from Juvenile Shoots Chilean J. Agric. Res.
García-Gonzáles,Rolando; Delgado,Miladys; González,Yailín; González,Aníbal; Garriga,Miguel; Caligari,Peter  D.S; Carrasco,Basilio; Quiroz,Karla.
Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium...
Tipo: Journal article Palavras-chave: Organogenesis; In vitro propagation; In vitro rooting; Plant growth regulators.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300005
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Molecular Tools for Rapid and Accurate Detection of Black Truffle (Tuber melanosporum Vitt.) in Inoculated Nursery Plants and Commercial Plantations in Chile Chilean J. Agric. Res.
Cordero,Cecilia; Cáceres,Pablo; González,Gloria; Quiroz,Karla; Carmen,Bravo; Ramírez,Ricardo; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
Truffle (Tuber melanosporum Vitt.) culture is an agroforestry sector in Chile of increasing interest due to the high prices that truffles fetch in the national market and the recent evidence that its commercial production is possible in Chilean climatic and soil conditions. In this study, the efficiency of three methods of DNA extraction from a mix of 5 g of soil and roots from both nursery and field plants of Quercus ilex L. mycorrhized with T. melanosporum were evaluated, and a simple and reproducible protocol was established. Detection of T. melanosporum was performed by the technique of cleaved amplified polymorphic sequence (CAPS) from amplicons generated with the primers ADL1 (5´-GTAACGATAAAGGCCATCTATAGG-3´) and ADL3 (5´-CGTTTTTCCTGAACTCTTCATCAC-3`),...
Tipo: Journal article Palavras-chave: Ectomycorrhizas; Molecular markers; PCR; CAPS; Ectomicorrizas; Marcadores moleculares; PCR; CAPS.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300022
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Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile Ciencia e Investigación Agraria
Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S...
Tipo: Journal article Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018
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In vitro culture of Luma chequen from vegetative buds Ciencia e Investigación Agraria
Mancilla,Héctor; Quiroz,Karla; Arencibia,Ariel; Carrasco,Basilio; García-Gonzales,Rolando.
H. Mancilla, K. Quiroz, A. Arencibia, B. Carrasco, and R. García-Gonzales. 2013. In vitro culture of Luma chequen from vegetative buds. Cien. Inv. Agr. 40(3): 609-615. Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritional and ornamental potential. However, its native habitat is deteriorating gradually, and it is suffering from the effects of fragmentation that is being caused by the conversion of forest land to agricultural land and the natural expansion of monocultural plantations of exotic species, such as Pinus radiata. The purpose of this work is to develop an effective procedure for establishing in vitro cultures of the native Chilean species L. chequen. Aseptic nodal...
Tipo: Journal article Palavras-chave: Aseptic nodal segments; In vitro culture; Myrtaceae family; Organogenesis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300013
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Plant tissue culture: Current status, opportunities and challenges Ciencia e Investigación Agraria
García-Gonzáles,Rolando; Quiroz,Karla; Carrasco,Basilio; Caligari,Peter.
In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products -including transgenic plants with several traits- to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive...
Tipo: Journal article Palavras-chave: In vitro; Micropropagation; Organogenesis; Plant bioreactors; Somatic embryogenesis; Transgenic plants.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300001
Registros recuperados: 5
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