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Genetic variability and diversity of mutant rice revealed by quantitative traits and molecular markers Agrociencia
Oladosu,Yusuff; Rafii,Mohd Y.; Abdullah,Norhani; Malek,Mohammad Abdul; Rahim,Harun A.; Hussin,Ghazali; Ismail,Mohd Razi; Latif,Mohammad Abdul; Kareem,Isiaka.
Genetic variability with mutagenic agents has been employed in plant breeding due to its significant contribution to the improvement of the existing rice genotypes by using genetically diverse parents. This study evaluated the genetic variability and diversity of mutant rice using quantitative traits and inter-simple sequence repeats (ISSR) molecular markers. A field experiment was carried out on M5 generation of 31 developed mutant lines and their parent (control). Morphological traits and 25 ISSR molecular markers were used as tools for determining cultivar identities and genetic diversity. The selected primers generated 443 clear polymorphic bands with an average number of 18 per primer. The bands were 85.10 % polymorphic. Un-weighted Pair Group of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant breeding; Genetic diversity; Molecular marker; Mutagenicity; Polymerase chain reaction (PCR).
Ano: 2015 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000300002
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Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding Bragantia
Miah,Gous; Rafii,Mohd Y.; Ismail,Mohd Razi; Puteh,Adam Bin; Rahim,Harun Abdul; Ashkani,Sadegh; Latif,Abdul.
The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes). Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1) for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blast inheritance; Microsatellite markers; BC2F1 population; Rice variety.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000100033
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