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Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pine stands in Portugal BJM
Ragonezi,Carla; Caldeira,A. Teresa; Rosário Martins,M.; Salvador,Cátia; Santos-Silva,Celeste; Ganhão,Elsa; Klimaszewska,Krystyna; Zavattieri,Amely.
Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pisolithus arhizus; Lactarius deliciosus; Pinus pinea; M13-PCR; ITS.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047
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Species richness and root colonization of arbuscular mycorrhizal fungi in Syngonanthus elegans, an endemic and threatened species from the Cerrado domain in Brazil Ciência e Agrotecnologia
Costa,Hesmael Antonio Orlandi; Stürmer,Sidney Luiz; Ragonezi,Carla; Grazziotti,Paulo Henrique; Grazziotti,Danielle Cristina Fonseca Santos; Silva,Enilson de Barros.
ABSTRACT Syngonanthus elegans is an endangered plant species occurring in the Brazilian Cerrado whose interaction with arbuscular mycorrhizal fungi (AMF) is poorly understood. The aim of this work was to evaluate the occurrence of AMF species and mycorrhizal colonization of S. elegans in two sampling areas named "Soberbo" stream (Soberbo) and "Parque Nacional das Sempre-Vivas" (Park), both found in Diamantina-MG, Brazil. In each area, one plot (100 x 100 m) was established, and roots and soil samples near the roots were collected from 10 plants in each plot. Further sampling included three specimens each of Loudetiopsis chrysothrix and Xyris sp.. Typical mycorrhizal colonization structures were observed in S. elegans roots, and colonization was measured at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal diversity; Star flowers; Rupestrian fields.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000300326
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Growth and Nutrition of Eucalypt Rooted Cuttings Promoted by Ectomycorrhizal Fungi in Commercial Nurseries Rev. Bras. Ciênc. Solo
Gandini,Andrezza Mara Martins; Grazziotti,Paulo Henrique; Rossi,Márcio José; Grazziotti,Danielle Cristina Fonseca Santos; Gandini,Elizzandra Marta Martins; Silva,Enilson de Barros; Ragonezi,Carla.
ABSTRACT Ectomycorrhizal fungi (EMF) may improve the adaptation of eucalypts saplings to field conditions and allow more efficient fertilizer use. The effectiveness of EMF inoculum application in promoting fungal colonization, plant growth, nutrient uptake, and the quality of rooted cuttings was evaluated forEucalyptus urophylla under commercial nursery conditions. For inoculated treatments, fertilization of the sapling substrate was reduced by 50 %. The experiment was carried out in a completely randomized design in a 4 × 4 factorial arrangement, wherein the factors were inoculum application rates of 0 (control), 5, 10, and 15 gel beads of calcium alginate containing the vegetative mycelium of Amanita muscaria, Elaphomyces antracinus, Pisolithus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Symbiosis; Plant nutrition; Cuttings production; Phosphorus; Nitrogen; Potassium.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000601554
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