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	Provedor de dados BJM (1) Autor Garshasbi,Maryam (1) Javani,Siamak (1) Moradi,Soheila (1) Ramazani,Ali (1) Sorouri,Rahim (1) Palavra-chave Brucella abortus (1) Human Brucellosis (1) PCR (1) Serologic tests (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2014 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran BJM Garshasbi,Maryam; Ramazani,Ali; Sorouri,Rahim; Javani,Siamak; Moradi,Soheila. Brucella is an intracellular pathogen capable of infecting animals and humans. The aim of this study was to identify Brucella spp in sera of high risk individuals by a polymerase chain reaction (PCR)-based method. A total of 180 patients suspected to have Brucellosis were examined by serological tests. To establish a PCR protocol for diagnosis of active brucellosis, DNA was extracted from the serum samples by using a commercial kit. PCR amplification was done for detection of Brocella DNA using BCSP31 target gene and IS711 locus. The PCR assay showed that an amplicon of 223 bp was obtained in 73.8% (133/180) of the tested sera using primers (B4/B5) derived from a gene encoding the 31-kDa Brucella abortus antigen. In another PCR, an amplicon of 498 bp was... Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Serologic tests; Brucella abortus; Human Brucellosis. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200022 Registros recuperados: 1 Primeira ... 1 ... Última

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