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Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation BJM
Ramos,Aline de Souza; Fiaux,Sorele Batista; Leite,Selma Gomes Ferreira.
Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)-1: sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 x 10(6) spores; moisture level, 55%. The temperature established was 28ºC. The fermentation under the selected conditions led to a 6-PP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 6-pentyl-α-pyrone; Solid-state fermentation; Experimental design.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400022
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Whole cells in enantioselective reduction of benzyl acetoacetate BJM
Ribeiro,Joyce Benzaquem; Ramos,Aline de Souza; Lopes,Raquel de Oliveira; Silva,Gabriela Veloso Vieira da; Souza,Rodrigo Octavio Mendonça Alves de.
The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotransformation; Chiral reduction; Β-ketoester; Kluyveromyces marxianus; Immobilization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300023
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Hydroxylation of 1,8-cineole by Mucor ramannianusand Aspergillus niger BJM
Ramos,Aline de Souza; Ribeiro,Joyce Benzaquem; Teixeira,Bruna Gomes; Ferreira,José Luiz Pinto; Silva,Jefferson Rocha de A.; Ferreira,Alexandre do Amaral; Souza,Rodrigo Octavio Mendonça Alves de; Amaral,Ana Claudia F..
The monoterpenoid 1,8-cineole is obtained from the leaves of Eucalyptus globulus and it has important biological activities. It is a cheap natural substrate because it is a by-product of the Eucalyptuscultivation for wood and pulp production. In this study, it was evaluated the potential of three filamentous fungi in the biotransformation of 1,8-cineole. The study was divided in two steps: first, reactions were carried out with 1,8-cineole at 1 g/L for 24 h; afterwards, reactions were carried out with substrate at 5 g/L for 5 days. The substrate was hydroxylated into 2-exo-hydroxy-1,8-cineole and 3-exo-hydroxy-1,8-cineole by fungi Mucor ramannianus and Aspergillus niger with high stereoselectivity. Trichoderma harzianum was also tested but no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotransformation monoterpene 1; 8-cineole Mucor ramannianus Aspergillus niger.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100261
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