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	Provedor de dados BJM (2) BJID (1) J. Venom. Anim. Toxins incl. Trop. Dis. (1) Autor Ramos,Daniela Fernandes (4) Silva,Pedro Eduardo Almeida da (3) Dellagostin,Odir Antônio (2) Borsuk,Sibele (1) Colvara,Wilson (1) Dellagostin,Odir (1) Fagundes,Michel Quevedo (1) Matthiensen,Alexandre (1) McFadden,Johnjoe (1) Mendum,Tom (1) Seixas,Fabiana Kommling (1) Silva,Ana Bárbara Scholante (1) Tavares,Lucas (1) Trindade,Gilma Santos (1) Votto,Ana Paula Souza de (1) Yunes,João Sarkis (1) von Groll,Andrea (1) Mais... Palavra-chave Genotyping (2) Mycobacterium bovis (2) Antimycobacterial agents (1) BCG vaccine (1) Bovine (1) Bovine tuberculosis (1) Clinical diagnosis (1) Cytotoxic activity (1) Diagnosis (1) Gene knockout techniques (1) Microcystins (1) Mycobacteria (1) Tuberculosis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2015 (1) 2014 (2) 2012 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Antimycobacterial and cytotoxicity activity of microcystins J. Venom. Anim. Toxins incl. Trop. Dis. Ramos,Daniela Fernandes; Matthiensen,Alexandre; Colvara,Wilson; Votto,Ana Paula Souza de; Trindade,Gilma Santos; Silva,Pedro Eduardo Almeida da; Yunes,João Sarkis. Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 μM and 0.06 μM. The high activity of M. aeruginosa hexanic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacteria; Antimycobacterial agents; Cytotoxic activity; Microcystins. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100315 Rational design of diagnostic and vaccination strategies for tuberculosis BJID Borsuk,Sibele; Seixas,Fabiana Kommling; Ramos,Daniela Fernandes; Mendum,Tom; McFadden,Johnjoe; Dellagostin,Odir. The development of diagnostic tests which can readily differentiate between vaccinated and tuberculosis-infected individuals is crucial for the wider utilization of bacillus Calmette-Guérin (BCG) as vaccine in humans and animals. BCG_0092 is an antigen that elicits specific delayed type hypersensitivity reactions similar in size and morphological aspects to that elicited by purified protein derivative, in both animals and humans infected with the tubercle bacilli. We carried out bioinformatics analyses of the BCG_0092 and designed a diagnostic test by using the predicted MHC class I epitopes. In addition, we performed a knockout of this gene by homologous recombination in the BCG vaccine strain to allow differentiation of vaccinated from infected... Tipo: Info:eu-repo/semantics/other Palavras-chave: Gene knockout techniques; Clinical diagnosis; BCG vaccine. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100012 Molecular typing of Mycobacterium bovis isolates: a review BJM Ramos,Daniela Fernandes; Tavares,Lucas; Silva,Pedro Eduardo Almeida da; Dellagostin,Odir Antônio. Mycobacterium bovis is the main causative agent of animal tuberculosis (TB) and it may cause TB in humans. Molecular typing of M. bovis isolates provides precise epidemiological data on issues of inter- or intra-herd transmission and wildlife reservoirs. Techniques used for typing M. bovis have evolved over the last 2 decades, and PCR-based methods such as spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) have been extensively used. These techniques can provide epidemiological information about isolates of M. Bovis that may help control bovine TB by indicating possible links between diseased animals, detecting and sampling outbreaks, and even demonstrating cases of laboratory cross-contamination between... Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Bovine; Diagnosis; Genotyping; Mycobacterium bovis. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200001 Molecular typing of Mycobacterium bovis isolated in the south of Brazil BJM Ramos,Daniela Fernandes; Silva,Ana Bárbara Scholante; Fagundes,Michel Quevedo; von Groll,Andrea; Silva,Pedro Eduardo Almeida da; Dellagostin,Odir Antônio. Bovine tuberculosis is a major infectious disease of the cattle. In this study, 85 M. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern Brazil, were evaluated using spoligotyping and VNTR. The strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the frequent movement of cattle between different properties. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine tuberculosis; Mycobacterium bovis; Genotyping. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200039 Registros recuperados: 4 Primeira ... 1 ... Última

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